运用时间分辨荧光光谱法研究氟化物的跨膜转运。

Université libre de Bruxelles (ULB), Engineering of Molecular NanoSystems, Avenue F.D. Roosevelt 50, CP165/64, B-1050 Brussels, Belgium.

Department of Chemistry and RECETOX, Faculty of Science, Masaryk University, Brno 62500, Czech Republic.

Chem Commun (Camb). 2023 Apr 4;59(28):4185-4188. doi: 10.1039/d3cc00897e.

Here we present a new method to monitor fluoride transmembrane transport into liposomes using a europium(III) complex. We take advantage of the long emission lifetime of this probe to measure the transport activity of a fluorescent transporter. The high sensitivity, selectivity, and versatility of the assay allowed us to study different types of fluoride transporters and unravel their mechanisms of action.

在这里，我们提出了一种使用铕（III）配合物监测氟离子跨膜转运到脂质体的新方法。我们利用该探针的长荧光寿命来测量荧光转运体的转运活性。该测定方法具有高灵敏度、选择性和多功能性，使我们能够研究不同类型的氟化物转运体，并揭示其作用机制。