Harris Orthopaedics Laboratory, Massachusetts General Hospital; Department of Orthopaedic Surgery, Harvard Medical School.
Harris Orthopaedics Laboratory, Massachusetts General Hospital.
J Vis Exp. 2023 Mar 3(193). doi: 10.3791/64641.
Ultrahigh molecular weight polyethylene (UHMWPE) is widely used in total joint arthroplasties as a load-bearing surface. Periprosthetic joint infections, the majority of which occur shortly after joint replacement, constitute almost 25% of total knee revision surgeries, and the complete eradication of bacterial infection poses a major challenge. A promising way to tackle this problem is to ensure the local sustained delivery of antibiotic concentrations sufficient to inhibit the bacteria to support routine systemic antibiotic prophylaxis. There is increased research into the development of efficient local drug delivery devices. Although established antibacterial testing methods for drugs can be used to test the antibacterial efficacy of drug-eluting materials, they are lacking in terms of providing real-time and longitudinal antibacterial efficacy data that can be correlated to the elution profiles of antibiotics from these devices. Here, we report a direct and versatile methodology to determine the antibacterial efficacy of antibiotic-eluting UHMWPE implants. This methodology can be used as a platform to avoid bacterial culture at each time point of a lengthy experiment and can also be adapted to other local drug delivery devices.
超高分子量聚乙烯（UHMWPE）作为一种承重表面，广泛应用于全关节置换术。假体周围关节感染，其中大多数发生在关节置换后不久，占全膝关节翻修手术的近 25%，而完全消除细菌感染是一个重大挑战。解决这个问题的一个有前途的方法是确保局部持续输送足够抑制细菌的抗生素浓度，以支持常规全身预防性使用抗生素。人们越来越关注开发有效的局部药物输送装置。尽管已经建立的药物抗菌测试方法可用于测试载药材料的抗菌功效，但这些方法缺乏能够与抗生素从这些装置中洗脱的洗脱曲线相关联的实时和纵向抗菌功效数据。在这里，我们报告了一种直接且通用的方法来确定载抗生素 UHMWPE 植入物的抗菌功效。该方法可作为一种平台，避免在冗长实验的每个时间点进行细菌培养，并且还可以适用于其他局部药物输送装置。
