含艰难梭菌 FliC 和 FliD 的重组融合蛋白疫苗可保护小鼠免受艰难梭菌感染。
Recombinant Fusion Protein Vaccine Containing Clostridioides difficile FliC and FliD Protects Mice against C. difficile Infection.
机构信息
Department of Molecular Medicine, Morsani College of Medicine, University of South Florida, Tampa, Florida, USA.
Department of Infectious Diseases and Global Health, Tufts University Cummings School of Veterinary Medicine, North Grafton, Massachusetts, USA.
出版信息
Infect Immun. 2023 Apr 18;91(4):e0016922. doi: 10.1128/iai.00169-22. Epub 2023 Mar 20.
Abstract
Bacterial flagella are involved in infection through their roles in host cell adhesion, cell invasion, auto-agglutination, colonization, the formation of biofilms, and the regulation and secretion of nonflagellar bacterial proteins that are involved in the virulence process. In this study, we constructed a fusion protein vaccine (FliCD) containing the Clostridioides difficile flagellar proteins FliC and FliD. The immunization of mice with FliCD induced potent IgG and IgA antibody responses against FliCD, protected mice against C. difficile infection (CDI), and decreased the C. difficile spore and toxin levels in the feces after infection. Additionally, the anti-FliCD serum inhibited the binding of C. difficile vegetative cells to HCT8 cells. These results suggest that FliCD may represent an effective vaccine candidate against CDI.
摘要
细菌鞭毛通过在宿主细胞黏附、细胞侵袭、自身聚集、定植、生物膜形成以及调节和分泌参与毒力过程的非鞭毛细菌蛋白等方面的作用而参与感染。在本研究中，我们构建了一种含有艰难梭菌鞭毛蛋白 FliC 和 FliD 的融合蛋白疫苗（FliCD）。用 FliCD 免疫小鼠可诱导针对 FliCD 的强烈 IgG 和 IgA 抗体应答，保护小鼠免受艰难梭菌感染（CDI），并降低感染后粪便中的艰难梭菌孢子和毒素水平。此外，抗-FliCD 血清抑制艰难梭菌营养细胞与 HCT8 细胞的结合。这些结果表明，FliCD 可能是一种有效的抗 CDI 疫苗候选物。
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