Bell R H, Place S, McCullough P, Ray M B, Rogers D H
Veterans Administration Medical Center, Department of Surgery, Cincinnati, OH 45220.
Int J Pancreatol. 1986 May;1(1):71-81. doi: 10.1007/BF02795241.
The technique of ex-vivo isolated pancreatic perfusion has been a valuable method for investigation of the physiology of the exocrine and endocrine pancreas. We have adapted the technique of isolated pancreatic perfusion for use in the Syrian golden hamster, an animal used widely in studies of pancreatic carcinogenesis. The technique involves surgical harvest of the pancreas with its aortic and portal venous blood supply intact and perfusion of the pancreas with a modified Krebs buffer at 37 degrees C. Physiological function of the perfused pancreas system was examined in 27 Syrian hamsters. In tests of endocrine function, the perfused pancreas responded by increasing insulin secretion within 1 min of elevating perfusate glucose concentration, and also secreted insulin promptly in response to 10 mM arginine. In exocrine studies, the flow of pancreatic juice was stimulated by the addition of 0.8 X 10(-9) M secretin to the perfusate, and amylase output was significantly increased by the addition of 0.2 X 10(-9) M cholecystokinin (CCK-8). The ex-vivo isolated perfused pancreas in the hamster thus appears to respond appropriately to physiological stimuli and is a valuable additional tool for studies of the hamster pancreas.
离体孤立胰腺灌注技术一直是研究胰腺外分泌和内分泌生理学的一种有价值的方法。我们对离体胰腺灌注技术进行了改进，使其适用于叙利亚金仓鼠，这种动物广泛用于胰腺癌发生的研究。该技术包括完整切除带有主动脉和门静脉血液供应的胰腺，并在37℃用改良的Krebs缓冲液灌注胰腺。在27只叙利亚仓鼠身上检查了灌注胰腺系统的生理功能。在内分泌功能测试中，灌注的胰腺在灌注液葡萄糖浓度升高后1分钟内通过增加胰岛素分泌做出反应，并且对10 mM精氨酸也迅速分泌胰岛素。在外分泌研究中，向灌注液中添加0.8×10(-9) M促胰液素可刺激胰液分泌，添加0.2×10(-9) M胆囊收缩素（CCK-8）可显著增加淀粉酶分泌量。因此，仓鼠体内的离体孤立灌注胰腺似乎对生理刺激有适当反应，是研究仓鼠胰腺的一种有价值的辅助工具。
