Key Laboratory of Agricultural Environment in Universities of Shandong, College of Resources and Environment, Shandong Agricultural University, 61 Daizong Road, Taian, 271018, People's Republic of China.
Department of Civil and Environmental Engineering, Hanyang University, Seongdong-Gu, Seoul, 04763, Republic of Korea.
Environ Geochem Health. 2023 Jul;45(7):4779-4793. doi: 10.1007/s10653-023-01491-1. Epub 2023 Mar 20.
Residual antibiotics (ABs) and heavy metals (HMs) are continuously released from soil, reflecting their intensive use and contamination of water and soil, posing an environmental problem of great concern. Relatively few studies exist of the functional diversity of soil microorganisms under the combined action of ABs and HMs. To address this deficiency, BIOLOG ECO microplates and the Integrated Biological Responses version 2 (IBRv2) method were used to comprehensively explore the effects of single and combined actions of copper (Cu) and enrofloxacin (ENR), oxytetracycline (OTC), and sulfadimidine (SM2) on the soil microbial community. The results showed that the high concentration (0.80 mmol/kg) compound group had a significant effect on average well color development (AWCD) and OTC showed a dose-response relationship. The results of IBRv2 analysis showed that the single treatment group of ENR or SM2 had a significant effect on soil microbial communities, and the IBRv2 of E1 was 5.432. Microbes under ENR, SM2, and Cu stress had more types of available carbon sources, and all treatment groups were significantly more enriched with microorganisms having D-mannitol and L-asparagine as carbon sources. This study confirms that the combined effects of ABs and HMs can inhibit or promote the function of soil microbial communities. In addition, this paper will provide new insights into IBRv2 as an effective method to evaluate the impacts of contaminants on soil health.
土壤中持续释放残留抗生素 (ABs) 和重金属 (HMs)，反映了它们的大量使用以及对水和土壤的污染，这是一个令人高度关注的环境问题。在 ABs 和 HMs 的共同作用下，土壤微生物功能多样性的相关研究相对较少。为了解决这一不足，本研究采用 BIOLOG ECO 微孔板和综合生物反应版本 2（IBRv2）方法，综合探讨了铜 (Cu) 和恩诺沙星 (ENR)、土霉素 (OTC) 和磺胺二甲嘧啶 (SM2) 单一及联合作用对土壤微生物群落的影响。结果表明，高浓度（0.80mmol/kg）复合组对平均颜色发展（AWCD）有显著影响，OTC 呈剂量-反应关系。IBRv2 分析结果表明，ENR 或 SM2 的单一处理组对土壤微生物群落有显著影响，E1 的 IBRv2 为 5.432。ENR、SM2 和 Cu 胁迫下的微生物具有更多类型的可用碳源，所有处理组对 D-甘露醇和 L-天冬酰胺作为碳源的微生物的富集程度明显更高。本研究证实 ABs 和 HMs 的联合效应可以抑制或促进土壤微生物群落的功能。此外，本文将为 IBRv2 作为评估污染物对土壤健康影响的有效方法提供新的见解。
