Key Laboratory of Agricultural Environment in Universities of Shandong, College of Resources and Environment, Shandong Agricultural University, Taian, 271018, PR China.
College of Plant Protection, Shandong Agricultural University, Taian, 271018, China.
Chemosphere. 2020 Jul;250:126161. doi: 10.1016/j.chemosphere.2020.126161. Epub 2020 Feb 10.
The widespread contamination of antibiotics and heavy metals results in imbalance in the ecosystem. However, the effect of the interaction between sulfamethazine (SM2) and copper (Cu) on soil enzymatic activities is unclear. Therefore, this study investigated the effect of single and combined artificial contamination of SM2 and Cu (0, 1.6 mmol kg Cu and 0, 0.05, 0.2, 0.8 mmol kg SM2) on soil enzymatic activities (urease, sucrose, phosphatase, and RubisCO). A single application of Cu at a concentration of 1.6 mmol kg inhibited the urease, phosphatase and sucrase activities, while a stimulating effect on RubisCO activity was observed on day 7, 21, and 28 of incubation. The individual application of SM2 at higher concentration exhibited significant inhibition of sucrase, phosphatase, and urease activities while a stimulatory effect on RubisCO activity was observed on day 14 and 21 of incubation. The combined contamination of SM2 and Cu significantly inhibited the activities of urease, sucrase, and phosphatase. The effect of combined contamination of SM2 and Cu on the activity of RubisCO was different. The analysis results of interaction types show that there are synergistic or antagonistic effects between Cu and SM2, and these effects can amplify or reduce the effect of Cu or SM2 on soil enzyme activities. Integrated biological responses version 2 (IBRv2) analysis showed that the combined contamination of Cu and SM2 had a greater inhibitory or stimulatory effect on soil enzyme activities than the single contamination of Cu and SM2, depending upon dose and time.
抗生素和重金属的广泛污染导致生态系统失衡。然而，磺胺甲恶唑（SM2）和铜（Cu）相互作用对土壤酶活性的影响尚不清楚。因此，本研究调查了单一和组合人工污染 SM2 和 Cu（0、1.6 mmol kg Cu 和 0、0.05、0.2、0.8 mmol kg SM2）对土壤酶活性（脲酶、蔗糖、磷酸酶和 RubisCO）的影响。1.6 mmol kg Cu 的单一应用抑制了脲酶、磷酸酶和蔗糖酶的活性，而在孵育的第 7、21 和 28 天观察到对 RubisCO 活性的刺激作用。较高浓度的 SM2 单独应用表现出对蔗糖酶、磷酸酶和脲酶活性的显著抑制作用，而在孵育的第 14 和 21 天观察到对 RubisCO 活性的刺激作用。SM2 和 Cu 的联合污染显著抑制了脲酶、蔗糖酶和磷酸酶的活性。SM2 和 Cu 联合污染对 RubisCO 活性的影响不同。相互作用类型的分析结果表明，Cu 和 SM2 之间存在协同或拮抗作用，这些作用可以放大或减少 Cu 或 SM2 对土壤酶活性的影响。整合生物反应版本 2（IBRv2）分析表明，Cu 和 SM2 的联合污染对土壤酶活性的抑制或刺激作用大于 Cu 和 SM2 的单一污染，具体取决于剂量和时间。
