State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, College of Animal Science and Technology, Guangxi University, Nanning 530004, China.
State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, College of Animal Science and Technology, Guangxi University, Nanning 530004, China.
Poult Sci. 2023 May;102(5):102605. doi: 10.1016/j.psj.2023.102605. Epub 2023 Feb 21.
In this study, the effects of naringin on hepatic yolk precursors formation and antioxidant capacity of Three-Yellow breeder hens during late laying period were evaluated. A total of 480 (54-wk-old) Three-Yellow breeder hens were randomly assigned to 4 groups (6 replicates of 20 hens): nonsupplemented control diet (C), and control diet supplemented with 0.1%, 0.2%, and 0.4% of naringin (N1, N2, and N3), respectively. Results showed that dietary supplemented with 0.1%, 0.2%, and 0.4% of naringin for 8 wk promoted the cell proliferation and attenuated the excessive fat accumulation in the liver. Compared with C group, increased concentrations of triglyceride (TG), total cholesterol (T-CHO), high-density lipoprotein cholesterol (HDL-C), and very low-density lipoprotein (VLDL), and decreased contents of low-density lipoprotein cholesterol (LDL-C) were detected in liver, serum and ovarian tissues (P < 0.05). After 8 wk of feeding with naringin (0.1%, 0.2%, and 0.4%), serum estrogen (E) level, expression levels of proteins and genes of estrogen receptors (ERs) increased significantly (P < 0.05). Meanwhile, naringin treatment regulated expression of genes related to yolk precursors formation (P < 0.05). Furthermore, dietary naringin addition increased the antioxidants, decreased the oxidation products, and up-regulated transcription levels of antioxidant genes in liver tissues (P < 0.05). These results indicated that dietary supplemented with naringin could improve hepatic yolk precursors formation and hepatic antioxidant capacity of Three-Yellow breeder hens during the late laying period. Doses of 0.2% and 0.4% are more effective than dose of 0.1%.
在这项研究中，评估了柚皮苷对三黄种母鸡产蛋后期卵黄前体形成和抗氧化能力的影响。将 480 只（54 周龄）三黄种母鸡随机分为 4 组（每组 6 个重复，每个重复 20 只母鸡）：不添加对照饲料（C）和对照饲料分别添加 0.1%、0.2%和 0.4%柚皮苷（N1、N2 和 N3）。结果表明，连续 8 周饲粮添加 0.1%、0.2%和 0.4%柚皮苷促进了细胞增殖，减轻了肝脏脂肪过度堆积。与 C 组相比，肝、血清和卵巢组织中甘油三酯（TG）、总胆固醇（T-CHO）、高密度脂蛋白胆固醇（HDL-C）和极低密度脂蛋白（VLDL）浓度升高，低密度脂蛋白胆固醇（LDL-C）含量降低（P < 0.05）。连续 8 周饲粮添加柚皮苷（0.1%、0.2%和 0.4%）后，血清雌激素（E）水平、雌激素受体（ERs）蛋白和基因表达水平显著升高（P < 0.05）。同时，柚皮苷处理调节了卵黄前体形成相关基因的表达（P < 0.05）。此外，饲粮添加柚皮苷增加了肝脏组织的抗氧化剂，减少了氧化产物，并上调了抗氧化基因的转录水平（P < 0.05）。这些结果表明，饲粮添加柚皮苷可改善三黄种母鸡产蛋后期的肝卵黄前体形成和肝脏抗氧化能力。0.2%和 0.4%的剂量比 0.1%的剂量更有效。
