Ren Rong, Bremner David H, Chen Wenling, Shi Anhua, Wang Tong, Wang Ying, Wang Chengji, Wu Junzi, Zhu Li-Min
College of Biological Science and Medical Engineering, Shanghai Engineering Research Center of Nano-Biomaterials and Regenerative Medicine, Donghua University, Shanghai 201620, China.
School of Science, Engineering and Technology, Abertay University, Kydd Building, Dundee DD1 1HG, Scotland, UK.
Int J Biol Macromol. 2023 May 31;238:124087. doi: 10.1016/j.ijbiomac.2023.124087. Epub 2023 Mar 20.
Gas therapy based on nitric oxide (NO) has emerged as a potential therapeutic approach for cancer, and in conjunction with multi-mode combination therapy, offers new possibilities for achieving significant hyperadditive effects. In this study, an integrated AI-MPDA@BSA nanocomposite for diagnosis and treatment was constructed for PDA based photoacoustic imaging (PAI) and cascade NO release. Natural NO donor L-arginine (L-Arg) and photosensitizer (PS) IR780 were loaded into mesoporous polydopamine (MPDA). Bovine serum albumin (BSA) was conjugated to the MPDA to increase the dispersibility and biocompatibility of the nanoparticles, as well as to serve as a gatekeeper controlling IR780 release from the MPDA pores. The AI-MPDA@BSA produced singlet oxygen (O) and converted it into NO through a chain reaction based on L-Arg, enabling a combination of photodynamic therapy and gas therapy. Moreover, due to the photothermal properties of MPDA, the AI-MPDA@BSA performed good photothermal conversion, which allowed photoacoustic imaging. As expected, both in vitro and in vivo studies have confirmed that the AI-MPDA@BSA nanoplatform has a significant inhibitory effect on cancer cells and tumors, and no apparent systemic toxicity or side effects were detected during the treatment period.
基于一氧化氮（NO）的气体疗法已成为一种潜在的癌症治疗方法，并且与多模式联合疗法相结合，为实现显著的超加性效应提供了新的可能性。在本研究中，构建了一种用于诊断和治疗的集成式AI-MPDA@BSA纳米复合材料，用于基于光声成像（PAI）的胰腺癌诊断及级联式NO释放。将天然NO供体L-精氨酸（L-Arg）和光敏剂（PS）IR780负载到介孔聚多巴胺（MPDA）中。牛血清白蛋白（BSA）与MPDA共轭，以提高纳米颗粒的分散性和生物相容性，并作为控制IR780从MPDA孔中释放的“守门人”。AI-MPDA@BSA产生单线态氧（O），并通过基于L-Arg的链式反应将其转化为NO，从而实现光动力疗法和气体疗法的联合。此外，由于MPDA的光热特性，AI-MPDA@BSA具有良好的光热转换性能，可实现光声成像。正如预期的那样，体外和体内研究均证实，AI-MPDA@BSA纳米平台对癌细胞和肿瘤具有显著的抑制作用，并且在治疗期间未检测到明显的全身毒性或副作用。
