Department of Veterinary and Animal Sciences, University of Copenhagen, Frederiksberg, Denmark.
Department of Veterinary and Animal Sciences, University of Copenhagen, Frederiksberg, Denmark.
J Nutr Biochem. 2023 Jun;116:109316. doi: 10.1016/j.jnutbio.2023.109316. Epub 2023 Mar 20.
Polyphenols are a class of bioactive plant compounds with health-promoting properties, however, the interactions between polyphenols and pathogen infection and their cumulative impact on inflammation and metabolic health are not well understood. Here, we investigated if a subclinical parasitic infection modulates the hepatic response to dietary polyphenol supplementation in a porcine model. Pigs were fed a diet with or without 1% grape proanthocyanidins (PAC) for 28 days. During the final 14 days of the experiment, half the pigs in each dietary group were inoculated with the parasitic nematode Ascaris suum. Serum biochemistry was measured and hepatic transcriptional responses were determined by RNA-sequencing coupled with gene-set enrichment analysis. A. suum infection resulted in reduced serum phosphate, potassium, sodium, and calcium, and increased serum iron concentrations. In uninfected pigs, PAC supplementation markedly changed the liver transcriptome including genes related to carbohydrate and lipid metabolism, insulin signaling, and bile acid synthesis. However, during A. suum infection, a separate set of genes were modulated by dietary PAC, indicating that the polyphenol-mediated effects were dependent on infection status. A. suum infection strongly influenced the expression of genes related to cellular metabolism, and, in contrast to the effects of PAC, these changes were mostly identical in both control-fed and PAC-fed pigs. Thus, the hepatic response to infection was mostly unaffected by concurrent polyphenol intake. We conclude that the presence of a commonly occurring parasite substantially influences the outcome of dietary polyphenol supplementation, which may have important relevance for nutritional interventions in populations where intestinal parasitism is widespread.
多酚是一类具有促进健康特性的生物活性植物化合物，但多酚与病原体感染之间的相互作用及其对炎症和代谢健康的累积影响尚不清楚。在这里，我们研究了亚临床寄生虫感染是否会调节猪模型中饮食多酚补充对肝脏的反应。猪食用含或不含 1%葡萄原花青素（PAC）的饮食 28 天。在实验的最后 14 天，每组饮食的一半猪被接种寄生线虫蛔虫。测量血清生化指标，并通过 RNA 测序结合基因集富集分析确定肝转录反应。蛔虫感染导致血清磷酸盐、钾、钠和钙减少，铁浓度增加。在未感染的猪中，PAC 补充显着改变了肝脏转录组，包括与碳水化合物和脂质代谢、胰岛素信号和胆汁酸合成相关的基因。然而，在蛔虫感染期间，饮食 PAC 调节了一组单独的基因，表明多酚介导的作用取决于感染状态。蛔虫感染强烈影响与细胞代谢相关的基因的表达，与 PAC 的作用相反，这些变化在对照喂养和 PAC 喂养的猪中基本相同。因此，感染时肝脏对多酚摄入的反应基本不受影响。我们得出结论，常见寄生虫的存在会极大地影响饮食多酚补充的结果，这对于肠道寄生虫广泛存在的人群中的营养干预可能具有重要意义。
