Chaves Nathalie Savedra Gomes, Janner Dieniffer Espinosa, Poetini Marcia Rósula, Fernandes Eliana Jardim, de Almeida Francielli Polet, Musachio Elize Aparecida Santos, Reginaldo Jocemara Corrêa, Dahleh Mustafa Munir Mustafa, de Carvalho Amarilis Santos, Leimann Fernanda Vitória, Gonçalves Odinei Hess, Ramborger Bruna Piaia, Roehrs Rafael, Prigol Marina, Guerra Gustavo Petri
Laboratório de Avaliações Farmacológicas e Toxicológicas Aplicadas às Moléculas Bioativas - LaftamBio, Universidade Federal do Pampa - Campus Itaqui, 97650-000 Itaqui, RS, Brazil; Programa de Pós-Graduação em Bioquímica, Universidade Federal do Pampa - Campus Uruguaiana, 97508-000 Uruguaiana, RS, Brazil.
Laboratório de Avaliações Farmacológicas e Toxicológicas Aplicadas às Moléculas Bioativas - LaftamBio, Universidade Federal do Pampa - Campus Itaqui, 97650-000 Itaqui, RS, Brazil.
Comp Biochem Physiol C Toxicol Pharmacol. 2023 Jun;268:109615. doi: 10.1016/j.cbpc.2023.109615. Epub 2023 Mar 20.
β-carotene-loaded nanoparticles improves absorption by increasing bioavailability. The Drosophila melanogaster model of Parkinson's disease must be helpful in investigating potential neuroprotective effects. Four groups of four-day-old flies were exposed to: (1) control; (2) diet containing rotenone (500 μM); (3) β-carotene-loaded nanoparticles (20 μM); (4) β-carotene-loaded nanoparticles and rotenone for 7 days. Then, the percentage of survival, geotaxis tests, open field, aversive phototaxis and food consumption were evaluated. At the end of the behaviors, the analyses of the levels of reactive species (ROS), thiobarbituric acid reactive substances (TBARS), catalase (CAT) and superoxide dismutase (SOD) activity was carried out, as well as an evaluation of the levels of dopamine and acetylcholinesterase (AChE) activity, in the head of flies. Nanoparticles loaded with β-carotene were able to improve motor function, memory, survival and also restored the oxidative stress indicators (CAT, SOD, ROS and TBARS), dopamine levels, AChE activity after exposure to rotenone. Overall, nanoparticles loaded with β-carotene showed significant neuroprotective effect against damage induced by the Parkinson-like disease model, emerging as a possible treatment. Overall, β-carotene-loaded nanoparticles presented significant neuroprotective effect against damage induced by model of Parkinson-like disease, emerging as a possible treatment.
负载β-胡萝卜素的纳米颗粒通过提高生物利用度来改善吸收。帕金森病的果蝇模型对于研究潜在的神经保护作用一定很有帮助。将四组四天大的果蝇暴露于：（1）对照组；（2）含有鱼藤酮（500 μM）的饮食；（3）负载β-胡萝卜素的纳米颗粒（20 μM）；（4）负载β-胡萝卜素的纳米颗粒和鱼藤酮，持续7天。然后，评估存活率、地趋性测试、旷场实验、厌恶性趋光性和食物消耗情况。在行为测试结束时，对果蝇头部的活性物质（ROS）、硫代巴比妥酸反应性物质（TBARS）、过氧化氢酶（CAT）和超氧化物歧化酶（SOD）活性水平进行分析，同时评估多巴胺水平和乙酰胆碱酯酶（AChE）活性。负载β-胡萝卜素的纳米颗粒能够改善运动功能、记忆力、存活率，还能恢复鱼藤酮暴露后的氧化应激指标（CAT、SOD、ROS和TBARS）、多巴胺水平以及AChE活性。总体而言，负载β-胡萝卜素的纳米颗粒对帕金森病样疾病模型诱导的损伤显示出显著的神经保护作用，有望成为一种可能的治疗方法。总体而言，负载β-胡萝卜素的纳米颗粒对帕金森病样疾病模型诱导的损伤呈现出显著的神经保护作用，有望成为一种可能的治疗方法。
