Laboratório de Avaliações Farmacológicas e Toxicológicas Aplicadas às Moléculas Bioativas - LaftamBio, Universidade Federal Do Pampa - Campus Itaqui, 97650-000, Itaqui, RS, Brazil; Programa de Pós-Graduação em Bioquímica, Universidade Federal do Pampa - Campus Uruguaiana, 97508-000, Uruguaiana, RS, Brazil.
Laboratório de Avaliações Farmacológicas e Toxicológicas Aplicadas às Moléculas Bioativas - LaftamBio, Universidade Federal Do Pampa - Campus Itaqui, 97650-000, Itaqui, RS, Brazil.
Chem Biol Interact. 2021 May 1;340:109431. doi: 10.1016/j.cbi.2021.109431. Epub 2021 Mar 11.
Parkinson's is a neurodegenerative disease, characterized by the loss of dopaminergic neurons, cholinergic alterations and oxidative damages. Lutein is widely known by its antioxidants properties. In the present study, we investigated whether lutein-loaded nanoparticles protects against locomotor damage and neurotoxicity induced by Parkinson's disease model in Drosophila melanogaster, as well as possible mechanisms of action. First, the nanoparticles were characterized by physicochemical methods, demonstrating that water affinity was improved by the encapsulation of lutein into the polymeric encapsulant matrix. The fruit flies of 1-4 days old were divided into four groups and exposed to a standard diet (control), a diet containing either rotenone (500 μM), lutein-loaded nanoparticles (6 μM) or rotenone (500 μM) and lutein-loaded nanoparticles (6 μM) for 7 days. The survival percentage was assessed, the flies were submitted to negative geotaxis, open field tasks and the determination of dopamine levels, tyrosine hydroxylase (TH) and acetylcholinesterase activities and oxidative stress indicators (superoxide dismutase, catalase, thiobarbituric acid reactive substances and glutathione S-transferase) were carried out. The exposure to lutein-loaded nanoparticles protected against locomotor damage and the decrease survival rate induced by rotenone, besides, it restored the dopamine levels, TH and acetylcholinesterase activities and oxidative stress indicators. These results provide evidence that lutein-loaded nanoparticles are an alternative treatment for rotenone-induced damage, and suggest the involvement of dopaminergic and cholinergic system and oxidative stress.
帕金森病是一种神经退行性疾病，其特征是多巴胺能神经元丧失、胆碱能改变和氧化损伤。叶黄素以其抗氧化特性而广为人知。在本研究中，我们研究了叶黄素负载的纳米粒子是否可以防止帕金森病模型诱导的果蝇运动损伤和神经毒性，以及可能的作用机制。首先，通过物理化学方法对纳米粒子进行了表征，证明叶黄素被包封在聚合物包封基质中提高了水亲和力。将 1-4 天大的果蝇分为四组，分别用标准饮食（对照）、含有鱼藤酮（500 μM）的饮食、负载叶黄素的纳米粒子（6 μM）或含有鱼藤酮（500 μM）和负载叶黄素的纳米粒子（6 μM）的饮食暴露 7 天。评估存活率，对果蝇进行负趋地性、旷场任务以及多巴胺水平、酪氨酸羟化酶（TH）和乙酰胆碱酯酶活性以及氧化应激指标（超氧化物歧化酶、过氧化氢酶、硫代巴比妥酸反应物质和谷胱甘肽 S-转移酶）的测定。暴露于负载叶黄素的纳米粒子可防止鱼藤酮诱导的运动损伤和存活率下降，此外，它还恢复了多巴胺水平、TH 和乙酰胆碱酯酶活性以及氧化应激指标。这些结果提供了证据表明，负载叶黄素的纳米粒子是治疗鱼藤酮诱导损伤的一种替代方法，并提示涉及多巴胺能和胆碱能系统以及氧化应激。
