Tadeo Francisco R, Agustí Javier, Merelo Paz, Talón Manuel
Centro de Genómica, Instituto Valenciano de Investigaciones Agrarias, Moncada, Valencia, Spain.
Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia-Consejo Superior de Investigaciones Científicas, Valencia, Spain.
Methods Mol Biol. 2023;2642:365-373. doi: 10.1007/978-1-0716-3044-0_19.
Plants display a great diversity of particular cell types that obviously perform functions and regulations that are essential for successful growth and development, whether under optimal or adverse conditions. The functions performed by each of these particular cell types must be associated with specific transcriptomic, proteomic, and metabolic profiles that cannot be disentangled by analyzing whole plant organs and tissues. Laser microdissection is a technique for the collection of specific cell types in plant organs and tissues comprising heterogeneous cell populations. It has been successfully used for physiological and molecular studies. Laser microdissection can be applied to any plant species as long as it is possible to reliably identify the cell types of interest. Here, we describe step by step, using citrus as a model plant, a fast, simple, easy to perform, and experimentally validated protocol to collect cells from the abscission zone, a specific tissue that is difficult to access and whose activity is important in the response of plants to adverse environmental conditions.
植物表现出多种多样的特定细胞类型，无论在最佳条件还是不利条件下，这些细胞类型显然都执行着对成功生长和发育至关重要的功能和调节作用。这些特定细胞类型各自执行的功能必定与特定的转录组、蛋白质组和代谢谱相关联，而通过分析整个植物器官和组织是无法理清这些关联的。激光显微切割是一种用于从包含异质细胞群体的植物器官和组织中收集特定细胞类型的技术。它已成功用于生理学和分子研究。只要能够可靠地识别出感兴趣的细胞类型，激光显微切割就可以应用于任何植物物种。在此，我们以柑橘作为模式植物，逐步描述一种快速、简单、易于操作且经过实验验证的方案，用于从脱落区收集细胞，脱落区是一种难以获取的特定组织，其活性在植物对不利环境条件的响应中很重要。
