Cai Min-Hua, Hu Ying-Kao, Li Ya-Xuan, Yan Yue-Ming
College of Life Science, Capital Normal University, Beijing 100037, China.
Yi Chuan. 2006 Oct;28(10):1325-9. doi: 10.1360/yc-006-1325.
To a large degree, the growth and development of plants depend on the organ and/or cell type-specific expression of genes. However, it can be difficult to obtain sufficient number of specific cells from any developmental stage to analyze expression of genes. Laser capture microdissection (LCM) is a novel technique that allows us to collect pure targeted cell subgroup or even a single cell quickly and precisely, thus the problem of tissue heterogeneity in molecular analysis can be resolved successfully. In this paper, the principles of LCM were introduced and the application of LCM in plant was summarized in gene expression analysis, proteomics and plant-microbe interaction. Meanwhile, possible directions of LCM in plant were put forward.
在很大程度上，植物的生长和发育取决于基因在器官和/或细胞类型中的特异性表达。然而，从任何发育阶段获取足够数量的特定细胞以分析基因表达可能会很困难。激光捕获显微切割（LCM）是一种新技术，它使我们能够快速、精确地收集纯的目标细胞亚群甚至单个细胞，从而成功解决分子分析中组织异质性的问题。本文介绍了LCM的原理，并总结了LCM在植物基因表达分析、蛋白质组学和植物-微生物相互作用中的应用。同时，提出了LCM在植物研究中的可能发展方向。
