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对同一患者的正常组织和病理组织进行单细胞转录组分析，揭示了结肠癌的进展。

Single-cell transcriptomic analysis of normal and pathological tissues from the same patient uncovers colon cancer progression.

作者信息

Sun Ruifang, Yang Yang, Lü Weidong, Yang Yanqi, Li Yulong, Liu Zhigang, Diao Dongmei, Wang Yang, Chang Su'e, Lu Mengnan, Jiang Qiuyu, Dai Bingling, Ma Xiaobin, Zhao Chang'an, Lü Moqi, Zhang Juan, Ding Caixia, Li Na, Zhang Jian, Xiao Zhengtao, Zhou Dangxia, Huang Chen

机构信息

Department of Oncology Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, 277 Yanta West Road, Xi'an, Shaanxi, People's Republic of China.

Department of Pathology, School of Basic Medical Sciences, Health Science Center, Xi'an Jiaotong University, 76 Yanta West Road, Xi'an, Shaanxi, People's Republic of China.

出版信息

Cell Biosci. 2023 Mar 21;13(1):62. doi: 10.1186/s13578-023-01002-w.

DOI:10.1186/s13578-023-01002-w
PMID:36944972
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10031920/
Abstract

The aim of the present study was to elucidate the evolutionary trajectory of colon cells from normal colon mucosa, to adenoma, then to carcinoma in the same microenvironment. Normal colon, adenoma and carcinoma tissues from the same patient were analyzed by single-cell sequencing, which perfectly simulated the process of time-dependent colon cancer due to the same microenvironment. A total of 22 cell types were identified. Results suggest the presence of dominant clones of same cells including C2 goblet cell, epithelial cell subtype 1 (Epi1), enterocyte cell subset 0 (Entero0), and Entero5 in carcinoma. Epi1 and Entero0 were Co-enriched in antibacterial and IL-17 signaling, Entero5 was enriched in immune response and mucin-type O-glycan biosynthesis. We discovered new colon cancer related genes including AC007952.4, NEK8, CHRM3, ANO7, B3GNT6, NEURL1, ODC1 and KCNMA1. The function of TBC1D4, LTB, C2CD4A, AND GBP4/5 in T cells needs to be clarified. We used colon samples from the same person, which provide new information for colon cancer therapy.

摘要

本研究的目的是阐明在同一微环境中结肠细胞从正常结肠黏膜到腺瘤再到癌的进化轨迹。通过单细胞测序分析来自同一患者的正常结肠、腺瘤和癌组织，由于微环境相同，这完美地模拟了时间依赖性结肠癌的进程。共鉴定出22种细胞类型。结果表明，癌组织中存在相同细胞的优势克隆，包括C2杯状细胞、上皮细胞亚型1（Epi1）、肠细胞亚群0（Entero0）和Entero5。Epi1和Entero0在抗菌和IL-17信号通路中共同富集，Entero5在免疫反应和粘蛋白型O-聚糖生物合成中富集。我们发现了新的结肠癌相关基因，包括AC007952.4、NEK8、CHRM3、ANO7、B3GNT6、NEURL1、ODC1和KCNMA1。TBC1D4、LTB、C2CD4A和GBP4/5在T细胞中的功能有待阐明。我们使用了来自同一人的结肠样本，这为结肠癌治疗提供了新信息。

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