Kutlu Zerrin, Halici Zekai, Gedikli Semin, Diyarbakir Busra, Civelek Maide Sena
Department of Biochemistry, Faculty of Pharmacy, Atatürk University, Erzurum, Turkey.
Faculty of Medicine, Department of Pharmacology, Atatürk University, Turkey.
Iran J Pharm Res. 2022 Aug 17;21(1):e127665. doi: 10.5812/ijpr-127665. eCollection 2022 Dec.
We aimed to evaluate the impact of the tryptanthrin (TRP) compound, with antimicrobial and anti-inflammatory effects, on the excisional wound (EW) model. In an EW model in mice, we tried to explain the possible effect of TRP through vascular endothelial growth factor (VEGF) and matrix metalloproteinase-9 (MMP-9) that contribute significantly to wound healing.
A total of 90 BALB-C female mice aged 6 - 8 weeks were used in the present study. Animals were randomly divided into five groups. After creating the EW model, three different doses (1, 2.5, 5 mg/kg) of TRP compound were applied topically for 14 days, and wound closure rates were measured on days 0, 3, and 7. Vascular endothelial growth factor and MMP-9 were evaluated on days 3, 7, and 14 on wound explants and on day 14 on serum samples by enzyme-linked immunosorbent assay. Histopathological analysis was performed on wound explants.
After the EW model creation, significant healing of the wound areas was observed in the groups for which TRP was applied, especially on the third day. Moreover, in groups that received the third dose of TRP, the wound closure rate was 94%. It was found that the wound areas were closed due to the increase in TRP dose. In line with wound healing, VEGF and MMP-9 levels gradually rose on the third and seventh days and decreased on the 14th day.
Tryptanthrin compound usage on the EW model increased wound healing and did not leave a scar after 14 days.
我们旨在评估具有抗菌和抗炎作用的靛玉红（TRP）化合物对切除伤口（EW）模型的影响。在小鼠的EW模型中，我们试图通过对伤口愈合有显著贡献的血管内皮生长因子（VEGF）和基质金属蛋白酶-9（MMP-9）来解释TRP的可能作用。
本研究共使用了90只6 - 8周龄的BALB-C雌性小鼠。动物被随机分为五组。建立EW模型后，局部应用三种不同剂量（1、2.5、5 mg/kg）的TRP化合物，持续14天，并在第0、3和7天测量伤口闭合率。通过酶联免疫吸附测定法在第3、7和14天对伤口外植体以及在第14天对血清样本评估血管内皮生长因子和MMP-9。对伤口外植体进行组织病理学分析。
建立EW模型后，在应用TRP的组中观察到伤口区域有显著愈合，尤其是在第三天。此外，在接受第三剂量TRP的组中，伤口闭合率为94%。发现伤口区域因TRP剂量增加而闭合。与伤口愈合一致，VEGF和MMP-9水平在第三天和第七天逐渐升高，在第14天下降。
在EW模型上使用靛玉红化合物可促进伤口愈合，且14天后不留疤痕。
