Kumar Mukesh, Has Canan, Lam-Kamath Khanh, Ayciriex Sophie, Dewett Deepshe, Bashir Mhamed, Poupault Clara, Schuhmann Kai, Knittelfelder Oskar, Raghuraman Bharath Kumar, Ahrends Robert, Rister Jens, Shevchenko Andrej
bioRxiv. 2023 Mar 6:2023.03.04.531088. doi: 10.1101/2023.03.04.531088.
The eye is a popular model to elucidate the molecular mechanisms that underlie the structure and function of the eye as well as the causes of retinopathies. For instance, the eye has been used to investigate the impacts of ageing and environmental stresses such as light-induced damage or dietary deficiencies. Moreover, large-scale screens have isolated genes whose mutation causes morphological and functional ocular defects, which includes key components of the phototransduction cascade. However, the proteome of the eye is poorly characterized. Here, we used GeLC-MS/MS to quantify 3516 proteins he adult eye and provide a generic and expandable resource for further genetic, pharmacological, and dietary studies.
眼睛是一种常用的模型，用于阐明构成眼睛结构和功能基础的分子机制以及视网膜病变的病因。例如，眼睛已被用于研究衰老和环境压力（如光诱导损伤或饮食缺乏）的影响。此外，大规模筛选已分离出其突变会导致形态和功能眼部缺陷的基因，其中包括光转导级联反应的关键成分。然而，眼睛的蛋白质组特征尚不明确。在这里，我们使用凝胶液相色谱串联质谱法（GeLC-MS/MS）对成年眼睛中的3516种蛋白质进行了定量分析，并为进一步的遗传学、药理学和饮食学研究提供了一个通用且可扩展的资源。
