Tristán Ana Isabel, González-Flores Encarnación, Salmerón Ana Del Mar, Abreu Ana Cristina, Caba Octavio, Jiménez-Luna Cristina, Melguizo Consolación, Prados José, Fernández Ignacio
Department of Chemistry and Physics, Research Centre CIAIMBITAL, University of Almería, Almería, Spain.
Instituto de Investigación Biosanitaria de Granada (ibs. GRANADA), Granada, Spain.
NMR Biomed. 2023 Mar 22:e4935. doi: 10.1002/nbm.4935.
We describe the use of nuclear magnetic resonance metabolomics to analyze blood serum samples from healthy individuals (n = 26) and those with metastatic colorectal cancer (CRC; n = 57). The assessment, employing both linear and nonlinear multivariate data analysis techniques, revealed specific metabolite changes associated with metastatic CRC, including increased levels of lactate, glutamate, and pyruvate, and decreased levels of certain amino acids and total fatty acids. Biomarker ratios such as glutamate-to-glutamine and pyruvate-to-alanine were also found to be related to CRC. The study also found that glutamate was linked to progression-free survival and that both glutamate and 3-hydroxybutyrate were risk factors for metastatic CRC. Additionally, gas chromatography coupled to flame-ionization detection was utilized to analyze the fatty acid profile and pathway analysis was performed on the profiled metabolites to understand the metabolic processes involved in CRC. A correlation was also found between the presence of certain metabolites in the blood of CRC patients and certain clinical features.
我们描述了利用核磁共振代谢组学分析健康个体（n = 26）和转移性结直肠癌患者（CRC；n = 57）的血清样本。该评估采用线性和非线性多变量数据分析技术，揭示了与转移性结直肠癌相关的特定代谢物变化，包括乳酸、谷氨酸和丙酮酸水平升高，以及某些氨基酸和总脂肪酸水平降低。还发现谷氨酸与谷氨酰胺的比值以及丙酮酸与丙氨酸的比值等生物标志物比率与结直肠癌有关。该研究还发现谷氨酸与无进展生存期相关，并且谷氨酸和3-羟基丁酸都是转移性结直肠癌的危险因素。此外，利用气相色谱-火焰离子化检测分析脂肪酸谱，并对分析的代谢物进行通路分析，以了解结直肠癌涉及的代谢过程。还发现结直肠癌患者血液中某些代谢物的存在与某些临床特征之间存在相关性。
