对iLID的无序连接改善了模型膜上的光开关蛋白图案。
A disordered tether to iLID improves photoswitchable protein patterning on model membranes.
作者信息
Di Iorio Daniele, Bergmann Johanna, Higashi Sayuri L, Hoffmann Arne, Wegner Seraphine V
机构信息
Institute of Physiological Chemistry and Pathobiochemistry, University of Münster, Germany.
出版信息
Chem Commun (Camb). 2023 Apr 6;59(29):4380-4383. doi: 10.1039/d3cc00709j.
PMID:36946614
Abstract
Reversible protein patterning on model membranes is important to reproduce spatiotemporal protein dynamics . An engineered version of iLID, disiLID, with a disordered domain as a membrane tether improves the recruitment of Nano under blue light and the reversibility in the dark, which enables protein patterning on membranes with higher spatiotemporal precision.
摘要
在模型膜上实现可逆的蛋白质图案化对于重现时空蛋白质动力学至关重要。一种经过工程改造的iLID版本，即disiLID，其带有一个无序结构域作为膜系链，可在蓝光下改善Nano的招募，并在黑暗中提高可逆性，这使得能够以更高的时空精度在膜上进行蛋白质图案化。
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