State Key Laboratory of Luminescent Materials and Devices, Center for Aggregation-Induced Emission, South China University of Technology, Guangzhou, 510640, China.
State Key Laboratory of Luminescent Materials and Devices, Center for Aggregation-Induced Emission, South China University of Technology, Guangzhou, 510640, China.
Biomaterials. 2019 May;203:43-51. doi: 10.1016/j.biomaterials.2019.03.002. Epub 2019 Mar 2.
The interplay of lipid droplets (LDs) and lysosome plays an important role in cell metabolism, and the visualization of this process can provide useful information of organelle communication and function. However, fluorescent bioprobes based on organic fluorophores that can respond to LD-lysosome interplay are much rare. Herein, fluorescent bioprobes with high photostability, excellent biocompatibility and intracellular polarity sensitivity are achieved by encapsulating a new red fluorogenic molecule TPA-BTTDO within polymeric matrix (DSPE-PEG). They can sequentially localize in lysosome and LDs with red and cyan emissions, respectively. By monitoring the emission color change, the interesting dynamic processes of the probes escaping from lysosome and then enriching in LDs, and finally returning to lysosome after LDs consumption are visualized. In addition, the tracing of dynamic movement and consumption of LDs is realized by the probes with a high signal-to-noise ratio. The unique labeling behaviors and distinguished dual emissions of the probes in LDs and lysosome make them promising agents for fluorescence visualization studies of LD-lysosome related bioprocess and metabolism diseases.
脂滴（LDs）与溶酶体的相互作用在细胞代谢中起着重要作用，而对这一过程的可视化可以提供有关细胞器通讯和功能的有用信息。然而，基于可以响应 LD-溶酶体相互作用的有机荧光团的荧光生物探针非常罕见。在此，通过将新型红色荧光发生分子 TPA-BTTDO 封装在聚合物基质（DSPE-PEG）中，实现了具有高光稳定性、优异的生物相容性和细胞内极性敏感性的荧光生物探针。它们可以分别以红色和青色发射来依次定位于溶酶体和 LDs 中。通过监测发射颜色变化，可视化了探针从溶酶体逃逸然后在 LDs 中富集，以及 LDs 消耗后返回溶酶体的有趣动态过程。此外，通过具有高信噪比的探针实现了 LDs 动态运动和消耗的追踪。探针在 LDs 和溶酶体中的独特标记行为和明显的双重发射使它们成为用于荧光可视化研究 LD-溶酶体相关生物过程和代谢疾病的有前途的试剂。
