Department of Medical Microbiology, Erciyes University Medical Faculty, Kayseri, Turkey.
Department of Medical Microbiology, Erciyes University Medical Faculty, Kayseri, Turkey.
Diagn Microbiol Infect Dis. 2023 May;106(1):115915. doi: 10.1016/j.diagmicrobio.2023.115915. Epub 2023 Feb 7.
In this study, it was aimed to evaluate the molecular epidemiology, virulence factors, and antifungal susceptibility of clinical Candida inconspicua isolates. All isolates were identified by phenotypic methods and sequence analysis of ITS 1-2, D1/D2, EF-1 alpha. Proteinase, phospholipase, and esterase activities, biofilm formation, and antifungal susceptibilities were determined. All thirty isolates identified as Candida norvegensis by phenotypic methods were reidentified as C. inconspicua by sequence analysis, demonstrating the inadequacy of phenotypic methods to differentiate these 2 species. The gene regions examined in terms of determining evolutionary relatedness did not show intraspecies nucleotide variations. Therefore, different molecular approaches are needed to evaluate molecular epidemiology. Esterase, phospholipase, and biofilm formation were found to be positive in 100%, 100%, and 36.6% of the strains, respectively. The MIC/MIC values for fluconazole and flucytosine were found to be higher than the other tested antifungals, which should be taken into account in the treatment.
在这项研究中，旨在评估临床不明显念珠菌分离株的分子流行病学、毒力因子和抗真菌药物敏感性。所有分离株均通过表型方法和 ITS1-2、D1/D2、EF-1α序列分析进行鉴定。测定蛋白酶、磷脂酶和酯酶活性、生物膜形成和抗真菌药物敏感性。所有通过表型方法鉴定为挪威念珠菌的 30 株分离株，通过序列分析重新鉴定为不明显念珠菌，表明表型方法不足以区分这 2 个种。在确定进化相关性方面检查的基因区域没有显示种内核苷酸变异。因此，需要不同的分子方法来评估分子流行病学。酯酶、磷脂酶和生物膜形成在 100%、100%和 36.6%的菌株中分别呈阳性。氟康唑和氟胞嘧啶的 MIC/MIC 值高于其他测试的抗真菌药物，在治疗中应考虑到这一点。
