Dong Jianyue, Kong Linghong, Jiang Weiwei, Wang Qi, Chen Yun, Liu Hanping
MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou, 510631, China; Guangzhou Key Laboratory of Spectral Analysis and Functional Probes, College of Biophotonics, South China Normal University, Guangzhou, 510631, China.
MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou, 510631, China; Guangzhou Key Laboratory of Spectral Analysis and Functional Probes, College of Biophotonics, South China Normal University, Guangzhou, 510631, China.
Biochem Biophys Res Commun. 2023 May 14;656:46-52. doi: 10.1016/j.bbrc.2023.03.027. Epub 2023 Mar 11.
Full-thickness skin wounds still represent a challenge for clinical treatment. Adipose-derived stem cells (ADSCs) therapy is a promising approach to achieve efficient healing in skin wounds. The excellent cell scaffold can promote proliferation, differentiation and paracrine of ADSCs in wound microenvironment, and is a key factor in ADSCs application. Herein, we first prepared the composite hydrogel with decellularized adipose tissue (DAT) and tremella polysaccharide (TPS), and loaded insulin (INS) into the DAT/TPS composite hydrogel (DAT/TPS-gel) to fabricate an efficient carrier for ADSCs in treating skin wound. Our study showed that INS modified DAT/TPS-gel (INS-DAT/TPS-gel) can promote the proliferation, differentiation and paracrine of ADSCs. INS-DAT/TPS-gel laden with ADSCs (ADSCs/INS-DAT/TPS-gel) effectively facilitated the skin wound healing in SD rats. These findings indicated that INS-DAT/TPS-gel was an effective scaffold for ADSCs transplantation, and ADSCs/INS-DAT/TPS-gel provides a potential strategy for the treatment of skin wounds.
全层皮肤伤口的临床治疗仍然是一项挑战。脂肪来源干细胞（ADSCs）疗法是实现皮肤伤口有效愈合的一种有前景的方法。优良的细胞支架能够促进ADSCs在伤口微环境中的增殖、分化和旁分泌，是ADSCs应用的关键因素。在此，我们首先制备了脱细胞脂肪组织（DAT）与银耳多糖（TPS）的复合水凝胶，并将胰岛素（INS）负载到DAT/TPS复合水凝胶（DAT/TPS-gel）中，以构建一种用于ADSCs治疗皮肤伤口的高效载体。我们的研究表明，INS修饰的DAT/TPS-gel（INS-DAT/TPS-gel）能够促进ADSCs的增殖、分化和旁分泌。负载ADSCs的INS-DAT/TPS-gel（ADSCs/INS-DAT/TPS-gel）有效促进了SD大鼠皮肤伤口的愈合。这些发现表明，INS-DAT/TPS-gel是ADSCs移植的有效支架，而ADSCs/INS-DAT/TPS-gel为皮肤伤口的治疗提供了一种潜在策略。
