IMEM-BRT Group, Departament d'Enginyeria Química, EEBE, Universitat Politècnica de Catalunya, C/ Eduard Maristany, 10-14, 08019 Barcelona, Spain; IITEMA-CONICET, Departamento de Química, Facultad de Ciencias Exactas Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Agencia Postal Nro. 3, X5804BYA Río Cuarto, Córdoba, Argentina.
IMEM-BRT Group, Departament d'Enginyeria Química, EEBE, Universitat Politècnica de Catalunya, C/ Eduard Maristany, 10-14, 08019 Barcelona, Spain; Barcelona Research Center in Multiscale Science and Engineering, Universitat Politècnica de Catalunya, 08930 Barcelona, Spain.
Int J Biol Macromol. 2023 May 31;238:124117. doi: 10.1016/j.ijbiomac.2023.124117. Epub 2023 Mar 21.
This work proposes a microfibers-hydrogel assembled composite as delivery vehicle able to combine into a single system both burst and prolonged release of lactate. The prolonged release of lactate has been achieved by electrospinning a mixture of polylactic acid and proteinase K (26.0 mg of proteinase K and 0.99 g of PLA dissolved in 6 mL of 2:1 chloroform:acetone in the optimal case), which is a protease that catalyzes the degradation of polylactic acid into lactate. The degradation of microfibers into lactate reflects that proteinase K preserves its enzymatic activity even after the electrospinning process because of the mild operational conditions used. Besides, burst release is obtained from the lactate-loaded alginate hydrogel. The successful assembly between the lactate-loaded hydrogel and the polylactic acid/proteinase K fibers has been favored by applying a low-pressure (0.3 mbar at 300 W) oxygen plasma treatment, which transforms hydrophobic fibers into hydrophilic while the enzymatic activity is still maintained. The composite displays both fast (< 24 h) and sustained (> 10 days) lactate release, and allows the modulation of the release by adjusting either the amount of loaded lactate or the amount of active enzyme.
这项工作提出了一种微纤维-水凝胶组装复合材料作为输送载体，能够将乳酸的爆发性释放和持续性释放结合在一个单一的系统中。乳酸的持续性释放是通过静电纺丝一种聚乳酸和蛋白酶 K 的混合物来实现的（在最佳情况下，将 26.0mg 的蛋白酶 K 和 0.99g 的 PLA 溶解在 6ml2:1 的氯仿：丙酮中），蛋白酶 K 能催化聚乳酸降解为乳酸。微纤维降解为乳酸反映了蛋白酶 K 即使在静电纺丝过程后仍保持其酶活性，这是由于使用了温和的操作条件。此外，从负载乳酸的海藻酸钠水凝胶中获得了爆发性释放。通过施加低压（0.3 毫巴在 300 瓦）的氧气等离子体处理，有利于负载乳酸的水凝胶与聚乳酸/蛋白酶 K 纤维之间的组装，该处理将疏水性纤维转化为亲水性纤维，同时保持酶活性。该复合材料具有快速（<24 小时）和持续（>10 天）的乳酸释放，并允许通过调节负载的乳酸量或活性酶的量来调节释放。
