Àlvarez Gerard, Arredondo Alexandre, Isabal Sergio, Teughels Wim, Laleman Isabelle, Contreras María José, Isbej Lorena, Huapaya Enrique, Mendoza Gerardo, Mor Carolina, Nart José, Blanc Vanessa, León Rubén
Department of Microbiology, DENTAID Research Center, Cerdanyola del Vallès, Spain.
Department of Oral Health Sciences, KU Leuven, Belgium & Dentistry, University Hospitals Leuven, Leuven, Belgium.
J Oral Microbiol. 2023 Mar 19;15(1):2188630. doi: 10.1080/20002297.2023.2188630. eCollection 2023.
Our aim was to compare the prevalence and load of nine pathobionts in subgingival samples of healthy individuals and periodontitis patients from four different countries.
Five hundred and seven subgingival biofilm samples were collected from healthy subjects and periodontitis patients in Belgium, Chile, Peru and Spain. The prevalence and load of and were measured by quantitative PCR.
The association with periodontitis of all species, except for , was confirmed in all countries but Peru, where only and were found to be significantly associated. Moreover, most species showed higher loads at greater CAL and PPD, but not where there was BOP. Through Principal Component Analysis, samples showed clearly different distributions by diagnosis, despite observing a smaller separation in Peruvian samples.
Unlike prevalence, relative load was found to be a reliable variable to discriminate the association of the species with periodontitis. Based on this, and may be biomarkers of disease in Belgium, Chile and Spain, due to their significantly higher abundance in periodontitis patients.
我们的目的是比较来自四个不同国家的健康个体和牙周炎患者龈下样本中九种致病共生菌的患病率和载量。
从比利时、智利、秘鲁和西班牙的健康受试者和牙周炎患者中收集了507份龈下生物膜样本。通过定量PCR测量[具体菌种]的患病率和载量。
除了[某种菌种]外，所有菌种与牙周炎的关联在除秘鲁外的所有国家均得到证实，在秘鲁仅发现[两种菌种]与牙周炎有显著关联。此外，大多数菌种在附着丧失（CAL）和探诊深度（PPD）较大时载量更高，但在有探诊出血（BOP）的情况下并非如此。通过主成分分析，尽管秘鲁样本的分离较小，但样本按诊断显示出明显不同的分布。
与患病率不同，发现相对载量是区分菌种与牙周炎关联的可靠变量。基于此，[两种菌种]在比利时、智利和西班牙可能是疾病的生物标志物，因为它们在牙周炎患者中的丰度显著更高。
