Abu Fanas Salem, Brigi Carel, Varma Sudhir Rama, Desai Vijay, Senok Abiola, D'souza Jovita
Ajman University, College of Dentistry, Department of Clinical Sciences, Ajman, United Arab Emirates; Center of Medical and Bio-allied Health Sciences research, Ajman University, Ajman, UAE.
Ajman University, College of Dentistry, Department of General Dentistry, Ajman, United Arab Emirates.
J Appl Oral Sci. 2021 May 17;29:e20200787. doi: 10.1590/1678-7757-2020-0787. eCollection 2021.
To define the subgingival microbial profile associated with Stage II generalized periodontitis using next-generation sequencing and to determine the relative abundance of novel periodontal pathogens and bacterial complexes.
Subgingival biofilm samples were collected from 80 subjects diagnosed with Stage II generalized periodontitis. Bacterial DNA was extracted, and 16S rRNA-based bacterial profiling via next-generation sequencing was carried out. The bacterial composition and diversity of microbial communities based on the age and sex of the patients were analyzed. The bacterial species were organized into groups: bacterial complexes (red, orange, purple, yellow, and green), novel periodontal pathogens, periodontal health-related species, and unclassified periodontal species. The results were analyzed and statistically evaluated.
The highest number of bacteria belonged to the phylum Bacteroidetes and Firmicutes. In terms of relative abundance, the orange complex represented 18.99%, novel bacterial species (Fretibacterium spp. and Saccharibacteria spp.) comprised 17.34%, periodontal health-related species accounted for 16.75% and unclassified periodontal species represented (Leptotrichia spp. and Selenomonas spp.) 15.61%. Novel periodontal pathogens had outweighed the periodontal disease-related red complex (5.3%). The one-sample z-test performed was statistically significant at p<0.05. The Beta diversity based on the unweighted UniFrac distance at the species level demonstrated a total variance of 15.77% based on age and 39.19% on sex, which was not statistically significant.
The bacterial species corresponding to the disease-related orange complex and novel periodontal pathogens are predominant in Stage II generalized periodontitis.
使用下一代测序技术确定与II期广泛性牙周炎相关的龈下微生物谱，并确定新型牙周病原体和细菌复合体的相对丰度。
从80例被诊断为II期广泛性牙周炎的受试者中收集龈下生物膜样本。提取细菌DNA，并通过下一代测序进行基于16S rRNA的细菌分析。分析了基于患者年龄和性别的微生物群落的细菌组成和多样性。将细菌种类分为几组：细菌复合体（红色、橙色、紫色、黄色和绿色）、新型牙周病原体、牙周健康相关物种和未分类的牙周物种。对结果进行分析和统计学评估。
细菌数量最多的属于拟杆菌门和厚壁菌门。在相对丰度方面，橙色复合体占18.99%，新型细菌物种（Fretibacterium spp.和Saccharibacteria spp.）占17.34%，牙周健康相关物种占16.75%，未分类的牙周物种（纤毛菌属和月形单胞菌属）占15.61%。新型牙周病原体的数量超过了与牙周疾病相关的红色复合体（5.3%）。进行的单样本z检验在p<0.05时具有统计学意义。基于物种水平的未加权UniFrac距离的β多样性显示，基于年龄的总方差为15.77%，基于性别的总方差为39.19%，无统计学意义。
与疾病相关的橙色复合体和新型牙周病原体对应的细菌物种在II期广泛性牙周炎中占主导地位。
