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.的血清总生物活性生物测定法

Bioassay for total serum bioactivity of .

作者信息

Na-Bangchang Kesara, Cheoymang Anurak, Muhamad Nadda, Kulma Inthuon

机构信息

Graduate Program in Bioclinical Sciences, Chulabhorn International College of Medicine, Thammasat University (Rangsit Campus), Pathumthani, Thailand.

出版信息

J Adv Pharm Technol Res. 2023 Jan-Mar;14(1):51-55. doi: 10.4103/japtr.japtr_431_22. Epub 2023 Jan 20.

DOI:10.4103/japtr.japtr_431_22
PMID:36950462
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10026330/
Abstract

The study aimed to establish a bioassay for total bioactivity of (AL) in human serum samples. Inhibition of bacterial growth ( ATCC 25923) was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. The calibration curve (0, 0.39, 0.78, 1.56, 3.13, 2.56, and 50 ng/μl) was linear with correlation coefficients >0.990. The limit of quantification (LOQ) was 1.66 mg/ml using 20-ml serum sample. The developed bioassay method meets the standard of the bioanalytical method for determination of serum bioactivity of AL.

摘要

该研究旨在建立一种用于检测人血清样本中（AL）总生物活性的生物测定法。使用3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴盐测定法评估对细菌生长（ATCC 25923）的抑制作用。校准曲线（0、0.39、0.78、1.56、3.13、2.56和50 ng/μl）呈线性，相关系数>0.990。使用20 ml血清样本时，定量限（LOQ）为1.66 mg/ml。所开发的生物测定方法符合测定AL血清生物活性的生物分析方法标准。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf02/10026330/d4485dea693d/JAPTR-14-51-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf02/10026330/bc52bed69225/JAPTR-14-51-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf02/10026330/d4485dea693d/JAPTR-14-51-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf02/10026330/bc52bed69225/JAPTR-14-51-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf02/10026330/d4485dea693d/JAPTR-14-51-g002.jpg

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