Marie Benjamin, Coulaud Romain, Boulangé-Lecomte Céline, Foucault Pierre, Lance Émilie, Duflot Aurélie, Xuereb Benoît
UMR 7245 CNRS-MNHN Molécules de Communication et Adaptation des Microorganismes, Muséum National d'Histoire Naturelle, Paris, France.
UNIHAVRE, UMR-I 02 INERIS-URCA-ULHN SEBIO, FR CNRS 3730 Scale, F-76063 Le Havre Cedex, France.
Data Brief. 2023 Mar 7;48:109038. doi: 10.1016/j.dib.2023.109038. eCollection 2023 Jun.
The prawn exhibits a large distribution (occurring along the Northeastern Atlantic coast to the Mediterranean), and has thus been found suitable as model organism valuable for various ecotoxicological studies. However, little is still known about the potential input of its metabolome and particularly concerning a potential molecular sexual dimorphism observable in the different tissues of this organism. In an ecotoxicological point of view, inter-sex and inter-organ differences of the metabolomes may introduce analytical bias and impact the robustness of the analysis and its interpretation. To explore such possibilities, we obtained qualitative metabolomic data from the analysis of different organs of mature male and female . We used ultra-high-performance liquid chromatography-electrospray ionization-high resolution tandem mass spectrometry (UHPLC-ESI-HRMS on positive mode) to characterize the 75%-extracted metabolome of both gills, hepatopancreas, nervous gland, muscle and gonads. The data were dereplicated using specific metabolomic software (MetaboScape 4) and 2,782 features were extracted, 1,720 of them being also analysed on MS/MS mode, supporting molecular networking investigations with Metgem 1.3.6. These metabolites were thus putatively identified using GNPS (Global Natural Product Social) Molecular Networking databases for de-novo annotation followed by manual curation of 84 metabolites. This data provides essential information on the important sexual dimorphism occurring at the molecular level in the different organs and supports further research on physiology and ecotoxicology in common European prawn.
对虾分布广泛（从东北大西洋沿岸到地中海均有出现），因此被认为是适用于各种生态毒理学研究的有价值的模式生物。然而，关于其代谢组的潜在信息，尤其是关于在该生物体不同组织中可观察到的潜在分子性别二态性，我们仍然知之甚少。从生态毒理学的角度来看，代谢组的性别间和器官间差异可能会引入分析偏差，并影响分析及其解释的稳健性。为了探索这种可能性，我们通过对成熟雄性和雌性对虾的不同器官进行分析，获得了定性代谢组学数据。我们使用超高效液相色谱 - 电喷雾电离 - 高分辨率串联质谱（正模式下的UHPLC - ESI - HRMS）来表征鳃、肝胰腺、神经腺、肌肉和性腺的75%提取代谢组。使用特定的代谢组学软件（MetaboScape 4）对数据进行去重复处理，提取了2782个特征峰，其中1720个也在MS/MS模式下进行了分析，以支持使用Metgem 1.3.6进行分子网络研究。因此，这些代谢物通过使用GNPS（全球天然产物社会）分子网络数据库进行从头注释，随后对84种代谢物进行人工整理，从而得到推定鉴定。这些数据提供了关于不同器官在分子水平上发生的重要性别二态性的基本信息，并支持对普通欧洲对虾的生理学和生态毒理学的进一步研究。
