Nash Paul B, Hemphill Melissa A, Barron James N
Department of Biological and Physical Sciences, Montana State University Billings, 1500 University Drive, Billings, MT, USA.
Department of Biology, University of West Florida, 11000 University Parkway, Pensacola, FL, USA.
Heliyon. 2023 Mar 8;9(3):e14368. doi: 10.1016/j.heliyon.2023.e14368. eCollection 2023 Mar.
The use of ketamine/xylazine for its anesthetic, anti-inflammatory, and anti-depressant properties could alter other medications and immune functions of the body. Thus, the current study explored how ketamine/xylazine affects the severity of influenza infection in BALB/c and C57Bl/6 mice, monitored for weight loss after intranasal inoculation with A/Puerto Rico/8/34 influenza virus. Mice were inoculated by using a micropipettor to insert 18 μL of control or a suspension of virus into each nostril and allowing the mouse to inhale the material. Several experiments were performed where groups of mice were treated with various combinations of virus and anesthesia and the results compared. Mice were weighed daily and monitored for other signs of illness. The experiments continued until the mice either regained their original weight or died (were euthanized when signs indicated non-recoverable status), which ranged from nine to twenty-three days. Anesthetized mice experienced more weight loss. Additionally, in experiments where the virus suspension was potent enough to lead to death, only mice that were anesthetized died.
氯胺酮/赛拉嗪因其麻醉、抗炎和抗抑郁特性的使用可能会改变身体的其他药物和免疫功能。因此，当前研究探讨了氯胺酮/赛拉嗪如何影响BALB/c和C57Bl/6小鼠流感感染的严重程度，在经鼻接种A/波多黎各/8/34流感病毒后监测体重减轻情况。通过使用微量移液器将18μL对照或病毒悬液插入每个鼻孔并让小鼠吸入该物质来对小鼠进行接种。进行了多项实验，对用病毒和麻醉的各种组合处理的小鼠组进行了比较。每天对小鼠称重并监测其他疾病迹象。实验持续进行，直到小鼠恢复其原始体重或死亡（当体征表明处于不可恢复状态时实施安乐死），这一过程持续9至23天。麻醉的小鼠体重减轻更多。此外，在病毒悬液效力足以导致死亡的实验中，只有麻醉的小鼠死亡。
