Tianjin Key Laboratory of Food Science and Health, School of Medicine, Nankai University, Tianjin, 300071, China.
School of Science, Hainan University, Haikou, 570228, China.
Small. 2023 Jun;19(26):e2300464. doi: 10.1002/smll.202300464. Epub 2023 Mar 22.
This work reports a portable, origami-type paper device with a plasmonic fluor-labeled microneedle sensing module for the multiplexed quantification of anemia biomarkers in whole blood. Sequential steps, including serum separation, target enrichment, and multiplexed readout by a gel imager, are rapidly accomplished with the flexible and highly integrated device. The microneedle array enabled efficient sampling of trace targets from ng mL to pg mL level. Combined with the plasmonic fluor label, the signal is improved by ≈7.6 folds compared with the flat substrate-based assay. The device is applied to simultaneously quantify hemoglobin (Hb), ferritin, folic acid (FA), and vitamin B (VB ), which are four anemia biomarkers distributed in different environments with different concentration ranges. Featured by the small sample volume (150 µL), short assay time (20 min), low cost (2 $), robust stability, and user-friendliness, the device is promising for the rapid and accurate diagnosis of anemia in real practice.
这项工作报道了一种便携式折纸型纸质装置，该装置带有等离子体荧光标记的微针传感模块，可用于全血中贫血生物标志物的多重定量检测。通过灵活且高度集成的装置，可以快速完成包括血清分离、目标富集以及通过凝胶成像仪进行多重读出的连续步骤。微针阵列能够从纳克毫升到皮克毫升级的痕量目标进行高效采样。与基于平面基底的检测相比，等离子体荧光标记将信号提高了约 7.6 倍。该装置用于同时定量检测血红蛋白 (Hb)、铁蛋白、叶酸 (FA) 和维生素 B (VB)，这四种贫血生物标志物分布在不同环境中，浓度范围也不同。该装置具有样本量小（150 μL）、检测时间短（20 分钟）、成本低（2 美元）、稳定性强、使用方便等特点，有望实现贫血的快速准确诊断，在实际应用中具有广阔的前景。
