sp. nov.，分离自猫口腔。

sp. nov., isolated from feline oral cavities.

机构信息

Department of Veterinary Science, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.

Management Department of Biosafety, Laboratory Animal, and Pathogen Bank, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.

出版信息

Int J Syst Evol Microbiol. 2023 Mar;73(3). doi: 10.1099/ijsem.0.005731.

DOI:10.1099/ijsem.0.005731

PMID:36951910

Abstract

Three bacterial strains, KC07075, KC07079 and KC07084, were isolated from the oral cavity of cats in 2007 in Japan. These strains were Gram-negative rods, exhibited gliding motility, grew in air with 5 % CO, and showed oxidase activity, but not catalase activity. The 16S rRNA gene sequences of the three strains were 100 % identical. The 16S rRNA gene sequence of strain KC07084 showed 92.1 and 91.9% identity to the type strains of and , respectively, and showed 89.3-91.6% identity to other species. The major cellular fatty acids of strain KC07084 were iso-C (58.4 %) and summed feature 11 (13.1 %). The G+C content of DNA from strain KC07084 was 33.7 mol%, and the genome size was 2.92 Mbp. Strains KC07075, KC07079 and KC07084 showed digital DNA-DNA hybridization values (dDDH) values of 99.9 % and average nucleotide identity (ANI) values of 99.98 % with each other, strain KC07084 had dDDH values of 18.7-28.2 % and ANI values of 67.12-72.30 % to the type strains of other species. All known species of the genus inhabiting the oral cavity of dogs and cats have catalase activity, but the three strains, including type strain KC07084, lacked catalase activity. These results of the phylogenetic analysis of the 16S rRNA gene sequence, biochemical characteristics, and dDDH and ANI values suggest that strain KC07084 represents a novel species. We propose the name sp. nov., with KC07084 as the type strain (=JCM 32682=DSM 107252).

摘要

2007 年，从日本猫的口腔中分离到三株细菌，分别命名为 KC07075、KC07079 和 KC07084。这些菌株均为革兰氏阴性杆菌，具有滑动运动性，在空气中（5% CO₂）生长，氧化酶活性阳性，过氧化氢酶活性阴性。三株菌的 16S rRNA 基因序列完全相同。KC07084 株的 16S rRNA 基因序列与和的模式株的相似度分别为 92.1%和 91.9%，与其他种的相似度为 89.3-91.6%。KC07084 株的主要细胞脂肪酸为 iso-C（58.4%）和特征脂肪酸 11（13.1%）。KC07084 株的 DNA G+C 含量为 33.7mol%，基因组大小为 2.92 Mbp。KC07075、KC07079 和 KC07084 株之间的数字 DNA-DNA 杂交值（dDDH）为 99.9%，平均核苷酸同一性（ANI）值为 99.98%，KC07084 株与其他种模式株的 dDDH 值为 18.7-28.2%，ANI 值为 67.12-72.30%。所有居住在犬猫口腔中的属种均具有过氧化氢酶活性，但包括模式株 KC07084 在内的这三株菌均缺乏过氧化氢酶活性。16S rRNA 基因序列的系统发育分析、生化特征以及 dDDH 和 ANI 值结果表明，KC07084 株代表了一个新种。我们建议将 KC07084 株命名为 sp. nov.，KC07084 株为该新种的模式株（=JCM 32682=DSM 107252）。