Yin Kevin, Cruz-Morales Pablo, Whitford Christopher M, Keasling Jay D
Joint BioEnergy Institute, Lawrence Berkeley National Laboratory, Emeryville, CA 94608, USA; Department of Plant and Microbial Biology, University of California, Berkeley, Berkeley, CA 94720, USA.
Joint BioEnergy Institute, Lawrence Berkeley National Laboratory, Emeryville, CA 94608, USA; Biological Systems and Engineering, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA; QB3 Institute, University of California, Berkeley, Berkeley, CA 94720, USA.
STAR Protoc. 2023 Mar 22;4(2):102190. doi: 10.1016/j.xpro.2023.102190.
Polycyclopropanated (POP) compounds show promise as fuels as their energy density can be greater than jet and rocket fuels in current use, but realizing their full potential requires significant development. This protocol guides the production of polycyclopropanated fatty acids in Streptomyces; POP production in another host remains to be demonstrated. This method can serve as a baseline for further development of POP as well as other polyketide products. For complete details on the use and execution of this protocol, please refer to Cruz-Morales et al. (2022)..
多环丙烷化（POP）化合物有望成为燃料，因为它们的能量密度可能高于目前使用的喷气燃料和火箭燃料，但要充分发挥其潜力还需要大量的研发工作。本方案指导链霉菌中多环丙烷化脂肪酸的生产；在另一种宿主中生产POP仍有待证明。该方法可作为进一步开发POP以及其他聚酮化合物产品的基线。有关本方案使用和执行的完整详细信息，请参考克鲁兹-莫拉莱斯等人（2022年）的研究。
