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开发用于非模式细菌沼泽红假单胞菌的合成生物学工具包的方案。

Protocol to develop a synthetic biology toolkit for the non-model bacterium R. palustris.

作者信息

Kathol Mark, Immethun Cheryl, Saha Rajib

机构信息

Department of Chemical and Biomolecular Engineering, University of Nebraska-Lincoln, Lincoln, NE, USA.

Department of Chemical and Biomolecular Engineering, University of Nebraska-Lincoln, Lincoln, NE, USA.

出版信息

STAR Protoc. 2023 Apr 26;4(2):102158. doi: 10.1016/j.xpro.2023.102158.

DOI:10.1016/j.xpro.2023.102158
PMID:37104094
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10154974/
Abstract

Numerous biology tools are developed to work for model organisms, which, however, do not work effectively in non-model organisms. Here, we present a protocol for developing a synthetic biology toolkit for Rhodopseudomonas palustris CGA009, a non-model bacterium with unique metabolic properties. We describe steps for introducing and characterizing biological devices in non-model bacteria, such as the utilization of fluorescence markers and RT-qPCR. This protocol may also be applicable for other non-model organisms. For complete details on the use and execution of this protocol, please refer to Immethun et al...

摘要

许多生物学工具是为模式生物开发的，然而，这些工具在非模式生物中并不能有效地发挥作用。在这里，我们展示了一种为沼泽红假单胞菌CGA009开发合成生物学工具包的方案，沼泽红假单胞菌CGA009是一种具有独特代谢特性的非模式细菌。我们描述了在非模式细菌中引入和表征生物装置的步骤，例如荧光标记物的利用和RT-qPCR。该方案也可能适用于其他非模式生物。有关该方案的使用和执行的完整详细信息，请参考Immethun等人的研究……

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a2b2/10154974/a6671a80517e/gr12.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a2b2/10154974/4e992f514867/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a2b2/10154974/633bb6926462/gr1.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a2b2/10154974/15ae76aadfe7/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a2b2/10154974/3aec5a444890/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a2b2/10154974/c320e2f3b40e/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a2b2/10154974/a5f45e4bc709/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a2b2/10154974/fe43e3881e75/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a2b2/10154974/438fa22943e6/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a2b2/10154974/feb4bfb0f5bb/gr9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a2b2/10154974/b7dd7e5c7b24/gr10.jpg
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