Pazzagli M, Magini A, Forti G, Bolelli G, Scarselli G, Noci I, Pellegrini S, Rodbard D, Serio M
Department of Clinical Physiopathology, University of Florence, Italy.
J Steroid Biochem. 1987;27(1-3):399-404. doi: 10.1016/0022-4731(87)90333-5.
The determination of the concentrations of estrone-3-glucuronide, pregnanediol-3-glucuronide and luteinizing hormone has been performed in early morning urine samples of 14 normal menstruating women using a timed and measured volume urine collection procedure. In order to investigate the variability of the urinary hormonal concentrations due to day-to-day differences in diuresis, the absolute hormonal concentrations have been corrected either for the urinary creatinine excretion or for the volume of urine voided during the night. The results demonstrate that both correction factors are able to reduce substantially the coefficient of variation values in comparison to the absolute hormonal concentrations. The urinary test of ovarian function has been performed in 11 infertile women affected by luteal insufficiency using the same procedure, and the hormonal profiles showed some alterations in both estrone-3-glucuronide and pregnanediol-3-glucuronide concentrations in comparison to the hormonal profiles of the normal subjects. Such alterations were significant in the single subject when integrated values of the hormonal data in defined time intervals were investigated.
采用定时定量收集尿液的方法，对14名正常月经周期女性的晨尿样本进行了雌酮-3-葡萄糖醛酸苷、孕二醇-3-葡萄糖醛酸苷和促黄体生成素浓度的测定。为了研究因每日尿量差异导致的尿激素浓度变异性，已根据尿肌酐排泄量或夜间排尿量对绝对激素浓度进行了校正。结果表明，与绝对激素浓度相比，这两个校正因子均能大幅降低变异系数值。采用相同方法对11名受黄体功能不全影响的不孕女性进行了卵巢功能尿检测，与正常受试者的激素谱相比，雌酮-3-葡萄糖醛酸苷和孕二醇-3-葡萄糖醛酸苷浓度的激素谱均出现了一些变化。当研究特定时间间隔内激素数据的综合值时，这些变化在单个受试者中具有显著性。
