一株需氧生长时对氯酸盐敏感的大肠杆菌K12突变体的特性分析。
Characterization of an Escherichia coli K12 mutant that is sensitive to chlorate when grown aerobically.
作者信息
Giordano G, Grillet L, Rosset R, Dou J H, Azoulay E, Haddock B A
出版信息
Biochem J. 1978 Nov 15;176(2):553-61. doi: 10.1042/bj1760553.
Abstract
Escherichia coli can normally grow aerobically in the presence of chlorate; however, mutants can be isolated that can no longer grow under these conditions. We present here the biochemical characterization of one such mutant and show that the primary genetic lesion occurs in the ubiquinone-8-biosynthetic pathway. As a consequence of this, under aerobic growth conditions the mutant is apparently unable to synthesize formate dehydrogenase, but can synthesize a Benzyl Viologen-dependent nitrate reductase activity. The nature of this activity is discussed.
摘要
大肠杆菌通常能够在氯酸盐存在的情况下进行有氧生长；然而，可以分离出在这些条件下不再能够生长的突变体。我们在此展示了一个此类突变体的生化特征，并表明主要的遗传损伤发生在泛醌-8生物合成途径中。因此，在有氧生长条件下，该突变体显然无法合成甲酸脱氢酶，但能够合成一种依赖苄基紫精的硝酸还原酶活性。讨论了这种活性的性质。
相似文献
1
Characterization of an Escherichia coli K12 mutant that is sensitive to chlorate when grown aerobically.一株需氧生长时对氯酸盐敏感的大肠杆菌K12突变体的特性分析。
Biochem J. 1978 Nov 15;176(2):553-61. doi: 10.1042/bj1760553.
2
Characterization of the membrane-bound nitrate reductase activity of aerobically grown chlorate-sensitive mutants of Escherichia coli K12.大肠杆菌K12需氧生长的氯酸盐敏感突变体膜结合硝酸还原酶活性的表征
FEBS Lett. 1978 Nov 15;95(2):290-4. doi: 10.1016/0014-5793(78)81013-8.
3
Causes of the inhibition of growth of Escherichia coli K12 by chlorate.氯酸盐抑制大肠杆菌K12生长的原因。
Can J Microbiol. 1974 Jan;20(1):65-74. doi: 10.1139/m74-011.
4
Role of quinones in electron transport to oxygen and nitrate in Escherichia coli. Studies with a ubiA- menA- double quinone mutant.醌类在大肠杆菌中向氧气和硝酸盐进行电子传递中的作用。对ubiA- menA-双醌突变体的研究。
Biochim Biophys Acta. 1977 Jul 7;461(1):84-100. doi: 10.1016/0005-2728(77)90071-8.
5
Assimilatory nitrate reductase in a chlorate-resistant mutant of Escherichia coli.大肠杆菌抗氯酸盐突变体中的同化型硝酸还原酶。
Z Allg Mikrobiol. 1976;16(7):543-50.
6
Nitrate reductase and the membrane composition of pleiotropic chlorate resistant mutants of Escherichia coli K-12.大肠杆菌K-12多效性抗氯酸盐突变体的硝酸还原酶与膜组成
Biochem Biophys Res Commun. 1979 Sep 12;90(1):86-91. doi: 10.1016/0006-291x(79)91593-6.
7
A mutant defective in electron transfer to nitrate in Escherichia coli K12.大肠杆菌K12中一种在向硝酸盐进行电子传递方面存在缺陷的突变体。
J Gen Microbiol. 1980 Mar;117(1):257-62. doi: 10.1099/00221287-117-1-257.
8
Molybdoenzyme biosynthesis in Escherichia coli: in vitro activation of purified nitrate reductase from a chlB mutant.大肠杆菌中钼酶的生物合成：来自chlB突变体的纯化硝酸还原酶的体外激活
J Bacteriol. 1992 Dec;174(24):7934-40. doi: 10.1128/jb.174.24.7934-7940.1992.
9
Effect of growth conditions on the synthesis of nitrate reductase components in chlorate resistant mutants of Escherichia coli K 12.生长条件对大肠杆菌K12氯酸盐抗性突变体中硝酸还原酶组分合成的影响。
Biochem Biophys Res Commun. 1979 Jul 27;89(2):659-63. doi: 10.1016/0006-291x(79)90680-6.
10
A novel method for isolating chlorate-resistant mutants of Escherichia coli K12 by anaerobic selection on a lactate plus fumarate medium.一种通过在乳酸加富马酸盐培养基上进行厌氧选择来分离大肠杆菌K12耐氯酸盐突变体的新方法。
J Gen Microbiol. 1976 Mar;93(1):173-6. doi: 10.1099/00221287-93-1-173.
引用本文的文献
1
Chromogenic assessment of the three molybdo-selenoprotein formate dehydrogenases in ..中三种钼硒蛋白甲酸脱氢酶的显色评估
Biochem Biophys Rep. 2015 Mar 30;1:62-67. doi: 10.1016/j.bbrep.2015.03.006. eCollection 2015 May.
2
The respiratory chains of Escherichia coli.大肠杆菌的呼吸链
Microbiol Rev. 1984 Sep;48(3):222-71. doi: 10.1128/mr.48.3.222-271.1984.
3
Two sites of oxygen control in induced synthesis of respiratory nitrate reductase in Escherichia coli.大肠杆菌中呼吸型硝酸还原酶诱导合成过程中的两个氧调控位点。
J Bacteriol. 1982 Mar;149(3):1142-5. doi: 10.1128/jb.149.3.1142-1145.1982.
4
Nitrate respiration in relation to facultative metabolism in enterobacteria.肠杆菌中与兼性代谢相关的硝酸盐呼吸作用
Microbiol Rev. 1988 Jun;52(2):190-232. doi: 10.1128/mr.52.2.190-232.1988.
本文引用的文献
1
Mutants of Escherichia coli requiring methionine or vitamin B12.需要甲硫氨酸或维生素B12的大肠杆菌突变体。
J Bacteriol. 1950 Jul;60(1):17-28. doi: 10.1128/jb.60.1.17-28.1950.
2
[The study and refinement of 2 methods permitting the measurement of bacterial nitrate reductase activity].[两种用于测量细菌硝酸还原酶活性的方法的研究与改进]
Ann Inst Pasteur (Paris). 1963 Feb;104:219-37.
3
[Measurement of formiate dehydrogenase activity in bacterial extracts].[细菌提取物中甲酸脱氢酶活性的测定]
Ann Inst Pasteur (Paris). 1969 Apr;116(4):433-47.
4
The role of shikimic acid in the biosynthesis of vitamin K2.莽草酸在维生素K2生物合成中的作用。
Biochem J. 1966 Jul;100(1):1-6. doi: 10.1042/bj1000001.
5
[Regulation of the synthesis of nitrate reductase in Escherichia coli K12].[大肠杆菌K12中硝酸还原酶合成的调控]
Ann Inst Pasteur (Paris). 1969 Oct;117(4):474-85.
6
Nitrate reductase complex of Escherichia coli K-12: participation of specific formate dehydrogenase and cytochrome b1 components in nitrate reduction.大肠杆菌K-12的硝酸还原酶复合体：特定甲酸脱氢酶和细胞色素b1组分在硝酸盐还原中的作用。
J Bacteriol. 1969 Sep;99(3):720-9. doi: 10.1128/jb.99.3.720-729.1969.
7
Biosynthesis of ubiquinone in Escherichia coli K-12: location of genes affecting the metabolism of 3-octaprenyl-4-hydroxybenzoic acid and 2-octaprenylphenol.大肠杆菌K-12中泛醌的生物合成：影响3-辛戊烯基-4-羟基苯甲酸和2-辛戊烯基苯酚代谢的基因定位。
J Bacteriol. 1969 Aug;99(2):450-8. doi: 10.1128/jb.99.2.450-458.1969.
8
[Study of chlorate resistant mutants of Escherichia coli K 12. I. Reconstitution in vitro of particulate nitrate reductase activity of Escherichia coli K 12].[大肠杆菌K12耐氯酸盐突变体的研究。I. 大肠杆菌K12颗粒状硝酸还原酶活性的体外重建]
Biochim Biophys Acta. 1969 Feb 11;171(2):238-52. doi: 10.1016/0005-2744(69)90157-0.
9
The role of a novel cytochrome b-containing nitrate reductase and quinone in the in vitro reconstruction of formate-nitrate reductase activity of E. coli.一种新型含细胞色素b的硝酸还原酶和醌在大肠杆菌甲酸-硝酸还原酶活性体外重建中的作用。
Biochem Biophys Res Commun. 1974 Dec 23;61(4):1234-41. doi: 10.1016/s0006-291x(74)80416-x.
10
Effects of molybdate, tungstate, and selenium compounds on formate dehydrogenase and other enzyme systems in Escherichia coli.钼酸盐、钨酸盐和硒化合物对大肠杆菌中甲酸盐脱氢酶及其他酶系统的影响。
J Bacteriol. 1972 Jun;110(3):1032-40. doi: 10.1128/jb.110.3.1032-1040.1972.
Zotero 插件