State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University, Yangling 712100, Shaanxi, China; Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northwest Region), Ministry of Agriculture, Yangling 712100, Shaanxi, China.
Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northwest Region), Ministry of Agriculture, Yangling 712100, Shaanxi, China; College of Life Sciences, Northwest A&F University, Yangling 712100, Shaanxi, China.
Plant Sci. 2023 Jun;331:111687. doi: 10.1016/j.plantsci.2023.111687. Epub 2023 Mar 22.
Stilbene and flavonoid are phytochemicals in plants and play an important role in plant disease resistance and human health. The regulation of stilbene and flavonoid synthesis in plants has been extensively studied at the transcriptional level, but translational and post-translational controls of stilbene and flavonoid biosynthesis are still poorly understood. In this study, a grape F-box E3 ubiquitin ligase VviKFB07 associated with the metabolism of stilbene and flavonoid was screened out with transcriptome. Overexpression of VviKFB07 in the Nicotiana tabacum resulted in a decrease in flavonol and anthocyanin content in corolla, and stable overexpression assays of VviKFB07 in grape callus promoted the accumulation of resveratrol. Subsequently, Yeast two-hybrid and bimolecular fluorescence complementation assays identified the physical interaction between VviKFB07 and VviCHSs proteins. In vivo experiments verified that VviKFB07 was involved in the ubiquitination and degradation of VviCHSs protein. Taken together, our findings clarify the role of ubiquitin ligase VviKFB07 in the synthesis of stilbene and flavonoid in grapes.
芪和类黄酮是植物中的植物化学物质，在植物的抗病性和人类健康中起着重要作用。植物中芪和类黄酮合成的调节已在转录水平上进行了广泛研究，但芪和类黄酮生物合成的翻译和翻译后控制仍知之甚少。在这项研究中，利用转录组筛选出与芪和类黄酮代谢有关的葡萄 F-box E3 泛素连接酶 VviKFB07。VviKFB07 在烟草中的过表达导致花瓣中类黄酮和花青素含量降低，葡萄愈伤组织中 VviKFB07 的稳定过表达测定促进了白藜芦醇的积累。随后，酵母双杂交和双分子荧光互补测定鉴定了 VviKFB07 与 VviCHSs 蛋白之间的物理相互作用。体内实验证实 VviKFB07 参与了 VviCHSs 蛋白的泛素化和降解。总之，我们的研究结果阐明了泛素连接酶 VviKFB07 在葡萄中芪和类黄酮合成中的作用。
