Molecular Ecology Group, Department of Ecology, Universität Innsbruck, Innsbruck, Austria; Division of Biological Science, Faculty of Science, Prince of Songkla University, Hat Yai, Thailand.
Department of Genetics, University of Cambridge, Cambridge, UK.
J Invertebr Pathol. 2023 Jun;198:107915. doi: 10.1016/j.jip.2023.107915. Epub 2023 Mar 21.
Wolbachia are known to cause reproductive manipulations and in some arthropod species, Wolbachia were reported to cause changes in gut microbiome. However, the effects of Wolbachia bacteria on the microbiomes of their hosts, including Drosophila flies, have not been fully accessed. Here, we checked the bacterial microbiome in guts of Wolbachia-uninfected and of Wolbachia-infected Drosophila nigrosparsa, both separated into a bleach-only (embryos bleached) and a gnotobiotic (embryos bleached and inoculated with bacteria) treatment. We observed a clear separation between the Wolbachia-infected and the Wolbachia-uninfected samples, and the infected samples had higher variation in alpha diversity than the uninfected ones. There were reductions in the abundances of Proteobacteria (Pseudomonadota), especially Acetobacter, in the infected samples of both treatments. These findings highlight that Wolbachia change the gut microbiome in D. nigrosparsa as well as that the interactions between Wolbachia and bacteria like Acetobacter need to be investigated.
已知沃尔巴克氏体可引起生殖操纵，在一些节肢动物物种中，报道称沃尔巴克氏体可引起肠道微生物组的变化。然而，沃尔巴克氏体细菌对其宿主（包括黑腹果蝇）微生物组的影响尚未被充分研究。在这里，我们检查了未感染和感染沃尔巴克氏体的黑腹果蝇肠道中的细菌微生物组，这两种果蝇都分为仅用漂白剂处理（胚胎漂白）和无菌处理（胚胎漂白并用细菌接种）。我们观察到感染和未感染的样本之间有明显的分离，感染样本的α多样性比未感染样本更高。两种处理的感染样本中，变形菌门（假单胞菌）的丰度，特别是醋酸杆菌属，都有所减少。这些发现表明，沃尔巴克氏体改变了黑腹果蝇的肠道微生物组，并且需要研究沃尔巴克氏体与醋酸杆菌等细菌之间的相互作用。
