The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, the Key Laboratory of Chemical Biology of Fujian Province, State Key Laboratory of Physical Chemistry of Solid Surfaces, Collaborative Innovation Centre of Chemistry for Energy Materials, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, China.
School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, China.
Angew Chem Int Ed Engl. 2023 May 15;62(21):e202215337. doi: 10.1002/anie.202215337. Epub 2023 Apr 17.
Isolation and analysis of tumor-derived extracellular vesicles (T-EVs) are important for clinical cancer management. Here, we develop a fluid multivalent magnetic interface (FluidmagFace) in a microfluidic chip for high-performance isolation, release, and protein profiling of T-EVs. The FluidmagFace increases affinity by 105-fold with fluidity-enhanced multivalent binding to improve isolation efficiency by 13.9 % compared with a non-fluid interface. Its anti-adsorption property and microfluidic hydrodynamic shear minimize contamination, increasing detection sensitivity by two orders of magnitude. Moreover, its reversibility and expandability allow high-throughput recovery of T-EVs for mass spectrometric protein analysis. With the chip, T-EVs were detected in all tested cancer samples with identification of differentially expressed proteins compared with healthy controls. The FluidmagFace opens a new avenue to isolation and release of targets for cancer diagnosis and biomarker discovery.
肿瘤衍生细胞外囊泡 (T-EV) 的分离和分析对于临床癌症管理非常重要。在这里，我们在微流控芯片中开发了一种流体多价磁界面 (FluidmagFace)，用于 T-EV 的高性能分离、释放和蛋白质分析。FluidmagFace 通过增强流体的多价结合提高了 105 倍的亲和力，与非流体界面相比，分离效率提高了 13.9%。其抗吸附特性和微流体剪切力最小化了污染，将检测灵敏度提高了两个数量级。此外，其可还原性和可扩展性允许高通量回收 T-EV 进行质谱蛋白质分析。使用该芯片，在所有测试的癌症样本中均检测到了 T-EV，并与健康对照相比鉴定出了差异表达的蛋白质。FluidmagFace 为癌症诊断和生物标志物发现的靶标分离和释放开辟了新途径。
