Department of Woman, Child and General and Specialized Surgery, University of Campania "Luigi Vanvitelli", Naples, Italy.
Department of Clinical Medicine and Surgery, University Federico II, Naples, Italy.
Pediatr Res. 2023 Sep;94(3):1111-1118. doi: 10.1038/s41390-023-02534-0. Epub 2023 Mar 23.
In children with an allergy to cow's milk proteins (CMA), the altered composition of intestinal microbiota influences the immune tolerance to milk proteins (CMP). This study aims to investigate the effect of probiotics on the phenotype and activation status of peripheral basophils and lymphocytes in a pediatric CMA cohort.
CMA children underwent 45 days of treatment with Bifidobacteria. The basophil degranulation and the immune phenotype of B cells, T helper cells, and regulatory T cells were analyzed in peripheral blood at diagnosis (T0), after a 45-day probiotic treatment (T1), and 45 days after the probiotic wash-out (T2).
We observed in probiotic-treated CMA patients a decrease in naive T lymphocytes. Among the CD3+ cell subsets, both naive and activated CD4+ cells resulted markedly reduced after taking probiotics, with the lowest percentages at T2. A decreased basophil degranulation was observed in response to all analyzed CMP at T1 compared to T0.
The probiotic treatment resulted in a decrease of circulating naive and activated CD4+ T cells, as well as degranulating basophils. These data suggest that the Bifidobacteria could have a beneficial effect in the modulation of oral tolerance to CMP.
ISRCTN69069358. URL of registration: https://www.isrctn.com/ISRCTN69069358 .
Probiotic treatment with Bifidobacteria induces a reduction of both naive and activated circulating CD4+ T cells in pediatric patients with cow's milk allergy (CMA). The probiotic supplementation induces a decreased basophil degranulation. The immunological tolerance persists even after 45 days of the probiotic wash-out. Bifidobacteria in vivo supplementation down-modulates the activation of innate and adaptive immunity in pediatric patients with cow's milk allergy. Bifidobacteria contribute to the development of immune tolerance in CMA patients.
在对牛奶蛋白（CMP）过敏的儿童中，肠道微生物群的改变组成会影响对牛奶蛋白的免疫耐受。本研究旨在调查益生菌对儿科 CMA 队列中外周嗜碱性粒细胞和淋巴细胞表型和激活状态的影响。
CMA 儿童接受双歧杆菌治疗 45 天。在诊断时（T0）、45 天益生菌治疗后（T1）和益生菌冲洗后 45 天（T2），分析外周血中的嗜碱性粒细胞脱颗粒和 B 细胞、辅助性 T 细胞和调节性 T 细胞的免疫表型。
我们观察到益生菌治疗的 CMA 患者幼稚 T 淋巴细胞减少。在 CD3+细胞亚群中，接受益生菌治疗后，幼稚和激活的 CD4+细胞明显减少，T2 时百分比最低。与 T0 相比，T1 时所有分析的 CMP 均观察到嗜碱性粒细胞脱颗粒减少。
益生菌治疗导致循环幼稚和激活的 CD4+T 细胞以及脱颗粒的嗜碱性粒细胞减少。这些数据表明，双歧杆菌可能对调节 CMP 的口服耐受性具有有益作用。
ISRCTN69069358。注册网址：https://www.isrctn.com/ISRCTN69069358。
双歧杆菌益生菌治疗可导致儿科牛奶过敏（CMA）患者循环幼稚和激活的 CD4+T 细胞减少。益生菌补充剂可诱导嗜碱性粒细胞脱颗粒减少。即使在益生菌冲洗后 45 天，免疫耐受仍持续存在。双歧杆菌体内补充可下调儿科 CMA 患者固有和适应性免疫的激活。双歧杆菌有助于 CMA 患者的免疫耐受发展。
