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低水平激光治疗对兔原位 Masquelet 技术诱导膜的影响。

Effect of low-level laser therapy on the membrane induced by the Masquelet technique at an orthotopic site in rabbits.

机构信息

MSc. Postgraduate Program in Animal Biotechnology - Department of Veterinary Surgery and Animal Reproduction - School of Veterinary Medicine and Animal Science - Universidade Estadual Paulista (UNESP) - Botucatu (SP), Brazil.

MSc, PhD. Department of Veterinary Surgery and Animal Reproduction - School of Veterinary Medicine and Animal Science - Universidade Estadual Paulista (UNESP) - Botucatu (SP), Brazil.

出版信息

Acta Cir Bras. 2021 Nov 22;36(10):e361003. doi: 10.1590/ACB361003. eCollection 2021.

DOI:10.1590/ACB361003
PMID:34817024
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8610207/
Abstract

PURPOSE

To evaluate the low-level laser therapy (LLLT) on the membrane induced by the Masquelet technique in rabbits.

METHODS

Twelve Norfolk rabbits at approximately 3 months of age were used. A 1-cm segmental defect was induced in both radii, which were filled with polymethylmethacrylate cylinder. LLLT was used postoperatively in the bone defect of one of the forelimbs every 48 hours for 15 days. Six rabbits were euthanatized on third and sixth postoperative weeks.

RESULTS

In both forelimbs, radiographs showed new bone growth from radius cut ends on the third postoperative week and more advanced stage on the sixth postoperative week. Ultrasound showed induced membrane one week after the surgery. Histologically, there were no significant differences in the semi-quantitative score of inflammation intensity, total number of blood vessels, bone metaplasia, and collagen. The average thicknesses were 2,050.17 and 1,451.96 μm for control membranes and 2,724.26 and 2,081.03 μm for irradiated membranes, respectively, on third and sixth postoperative weeks. Vascular endothelial growth factor A (VEGF-A) and platelet derived growth factor (PDGF) expression were present in the induced membranes of control and irradiated forelimbs, but there was no significant difference.

CONCLUSIONS

Based on assessment methods, it was not possible to demonstrate the effect of LLLT on the induced membrane.

摘要

目的

评估低水平激光疗法（LLLT）对 Masquelet 技术诱导膜的影响。

方法

使用 12 只大约 3 月龄的 Norfolk 兔。在双侧桡骨中造成 1cm 的节段性缺损，并用聚甲基丙烯酸甲酯柱填充。术后每 48 小时对一侧前肢的骨缺损进行 LLLT，共 15 天。术后第 3 和第 6 周，6 只兔子被安乐死。

结果

在双侧前肢，X 线片显示术后第 3 周桡骨断端有新骨生长，第 6 周有更先进的阶段。超声检查在手术后一周显示诱导膜。组织学上，炎症强度、血管总数、骨化生和胶原的半定量评分无显著差异。对照组和照射组的膜厚度分别为术后第 3 周的 2050.17μm 和 1451.96μm，第 6 周的 2724.26μm 和 2081.03μm。对照组和照射组前肢的诱导膜中均有血管内皮生长因子 A（VEGF-A）和血小板衍生生长因子（PDGF）表达，但无显著差异。

结论

根据评估方法，无法证明 LLLT 对诱导膜的影响。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33e7/8610207/22c18aebe600/1678-2674-acb-36-10-e361003-gf06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33e7/8610207/d7fb521f63a1/1678-2674-acb-36-10-e361003-gf01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33e7/8610207/6bb8ed1528fc/1678-2674-acb-36-10-e361003-gf02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33e7/8610207/616350d1936a/1678-2674-acb-36-10-e361003-gf03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33e7/8610207/44d5624053bc/1678-2674-acb-36-10-e361003-gf04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33e7/8610207/15e1a154d574/1678-2674-acb-36-10-e361003-gf05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33e7/8610207/22c18aebe600/1678-2674-acb-36-10-e361003-gf06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33e7/8610207/d7fb521f63a1/1678-2674-acb-36-10-e361003-gf01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33e7/8610207/6bb8ed1528fc/1678-2674-acb-36-10-e361003-gf02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33e7/8610207/616350d1936a/1678-2674-acb-36-10-e361003-gf03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33e7/8610207/44d5624053bc/1678-2674-acb-36-10-e361003-gf04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33e7/8610207/15e1a154d574/1678-2674-acb-36-10-e361003-gf05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33e7/8610207/22c18aebe600/1678-2674-acb-36-10-e361003-gf06.jpg

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