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早期肺腺癌中与淋巴管血管侵犯相关的细胞外囊泡相关 microRNA 特征。

Extracellular vesicle-associated microRNA signatures related to lymphovascular invasion in early-stage lung adenocarcinoma.

机构信息

Department of Thoracic Surgery, Tokyo Medical University, 6-7-1 Nishishinjuku, Shinjuku-ku, Tokyo, 160-0023, Japan.

Department of molecular and cellular medicine, Tokyo Medical University, Tokyo, Japan.

出版信息

Sci Rep. 2023 Mar 24;13(1):4823. doi: 10.1038/s41598-023-32041-5.

DOI:10.1038/s41598-023-32041-5
PMID:36964242
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10038982/
Abstract

Lymphovascular invasion (LVI) is a fundamental step toward the spread of cancer. Extracellular vesicles (EVs) promote cellular communication by shuttling cargo, such as microRNAs (miRNAs). However, whether EV-associated miRNAs serve as biomarkers for LVI remains unclear. This study aimed to identify EV-associated miRNAs related to LVI and validate the miRNA levels from patients with early-stage lung adenocarcinoma (LADC). Blood samples were collected from patients undergoing pulmonary resection for stage I LADC before surgery. The patients were classified into three groups according to the presence of LVI and postoperative recurrence. Serum-derived EVs in the derivation cohort were used for small RNA sequencing, while the selected LVI miRNA candidates were validated via real-time quantitative polymerase chain reaction using 44 patient and 16 healthy donor samples as the validation cohorts. Five miRNAs (miR-99b-3p, miR-26a-5p, miR-93-5p, miR-30d-5p, and miR-365b-3p) were assessed, and miR-30d-5p (p = 0.036) levels were significantly downregulated in the LVI-positive group. miR-30d-5p levels in healthy donors were lower than those in LADC patients. Patients with high miR-30d-5p levels had favorable survival compared to those with low miR-30d-5p levels. miR-30d-5p level in EVs may serve as a promising biomarker for detecting LVI in patients with early-stage LADC.

摘要

淋巴血管侵犯（LVI）是癌症扩散的基本步骤。细胞外囊泡（EVs）通过转运货物（如 microRNAs（miRNAs））来促进细胞间通讯。然而，EV 相关的 miRNAs 是否作为 LVI 的生物标志物尚不清楚。本研究旨在鉴定与 LVI 相关的 EV 相关 miRNAs，并验证早期肺腺癌（LADC）患者的 miRNA 水平。在手术前，采集接受肺切除术治疗 I 期 LADC 的患者的血液样本。根据 LVI 和术后复发的存在，将患者分为三组。在推导队列中使用血清衍生的 EV 进行小 RNA 测序，而通过实时定量聚合酶链反应使用 44 名患者和 16 名健康供体样本作为验证队列来验证选定的 LVI miRNA 候选物。评估了 5 个 miRNA（miR-99b-3p、miR-26a-5p、miR-93-5p、miR-30d-5p 和 miR-365b-3p），miR-30d-5p（p=0.036）水平在 LVI 阳性组中显著下调。健康供体中的 miR-30d-5p 水平低于 LADC 患者。miR-30d-5p 水平较高的患者的生存情况优于 miR-30d-5p 水平较低的患者。EV 中的 miR-30d-5p 水平可能作为检测早期 LADC 患者 LVI 的有前途的生物标志物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/49cb/10038982/7f4ddfa311e9/41598_2023_32041_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/49cb/10038982/1b6c8879c5bb/41598_2023_32041_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/49cb/10038982/6f24ec1c17ff/41598_2023_32041_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/49cb/10038982/3ef24079f8e5/41598_2023_32041_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/49cb/10038982/7c038b75f914/41598_2023_32041_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/49cb/10038982/7f4ddfa311e9/41598_2023_32041_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/49cb/10038982/1b6c8879c5bb/41598_2023_32041_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/49cb/10038982/6f24ec1c17ff/41598_2023_32041_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/49cb/10038982/3ef24079f8e5/41598_2023_32041_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/49cb/10038982/7c038b75f914/41598_2023_32041_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/49cb/10038982/7f4ddfa311e9/41598_2023_32041_Fig5_HTML.jpg

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本文引用的文献

1
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Clin Lung Cancer. 2023 May;24(3):e126-e133. doi: 10.1016/j.cllc.2022.12.006. Epub 2022 Dec 25.
2
Identification of a suitable endogenous control miRNA in bone aging and senescence.鉴定骨衰老和衰老过程中的合适内源性对照 miRNA。
Gene. 2022 Aug 15;835:146642. doi: 10.1016/j.gene.2022.146642. Epub 2022 Jun 11.
3
Exosomes and extracellular vesicles: Rethinking the essential values in cancer biology.
比较细胞外囊泡衍生 miRNA 的 3D-Gene 微阵列数据的预处理策略。
BMC Bioinformatics. 2024 Jun 20;25(1):221. doi: 10.1186/s12859-024-05840-4.
4
Non-coding RNAs in lung cancer: molecular mechanisms and clinical applications.肺癌中的非编码RNA：分子机制与临床应用
Front Oncol. 2023 Sep 8;13:1256537. doi: 10.3389/fonc.2023.1256537. eCollection 2023.
外泌体和细胞外囊泡：重新思考癌症生物学中的基本价值。
Semin Cancer Biol. 2021 Sep;74:79-91. doi: 10.1016/j.semcancer.2021.03.032. Epub 2021 Mar 31.
4
Extracellular Vesicles Are Key Regulators of Tumor Neovasculature.细胞外囊泡是肿瘤新生血管的关键调节因子。
Front Cell Dev Biol. 2020 Dec 9;8:611039. doi: 10.3389/fcell.2020.611039. eCollection 2020.
5
miR-486-3p mediates hepatocellular carcinoma sorafenib resistance by targeting FGFR4 and EGFR.miR-486-3p 通过靶向 FGFR4 和 EGFR 介导肝癌索拉非尼耐药。
Cell Death Dis. 2020 Apr 20;11(4):250. doi: 10.1038/s41419-020-2413-4.
6
Non-Exosomal and Exosomal Circulatory MicroRNAs: Which Are More Valid as Biomarkers?非外泌体和外泌体循环微小RNA：哪种作为生物标志物更有效？
Front Pharmacol. 2020 Jan 20;10:1500. doi: 10.3389/fphar.2019.01500. eCollection 2019.
7
Involvement of Extracellular Vesicles in Vascular-Related Functions in Cancer Progression and Metastasis.细胞外囊泡在癌症进展和转移中的血管相关功能中的作用。
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8
Exosomal let-7d-3p and miR-30d-5p as diagnostic biomarkers for non-invasive screening of cervical cancer and its precursors.外泌体 let-7d-3p 和 miR-30d-5p 作为宫颈癌及其前体非侵入性筛查的诊断生物标志物。
Mol Cancer. 2019 Apr 2;18(1):76. doi: 10.1186/s12943-019-0999-x.
9
An Insight into the Roles of MicroRNAs and Exosomes in Sarcoma.深入了解微小RNA和外泌体在肉瘤中的作用
Cancers (Basel). 2019 Mar 26;11(3):428. doi: 10.3390/cancers11030428.
10
Exosomal Metastasis‑Associated Lung Adenocarcinoma Transcript 1 Promotes Angiogenesis and Predicts Poor Prognosis in Epithelial Ovarian Cancer.外泌体转移相关肺腺癌转录本 1 促进血管生成并预测上皮性卵巢癌不良预后。
Int J Biol Sci. 2018 Nov 1;14(14):1960-1973. doi: 10.7150/ijbs.28048. eCollection 2018.