Hatipoğlu Abdulkerim, Baran Ayşe, Keskin Cumali, Baran Mehmet Fırat, Eftekhari Aziz, Omarova Sabina, Janas Dawid, Khalilov Rovshan, Adican Mehmet Tevfik, Kandemir Sevgi İrtegün
Department of Nutrition and Dietetics, Faculty of Health Sciences, Mardin Artuklu University, Mardin, Turkey.
Department of Biology, Graduate Education Institute, Mardin Artuklu University, Mardin, Turkey.
Environ Sci Pollut Res Int. 2023 Mar 24. doi: 10.1007/s11356-023-26499-z.
Silver nanoparticles (AgNPs) have several uses. Many scientists are working on producing AgNPs from plant extracts for use as biomedicines against drug-resistant bacteria and malignant cell lines. In the current study, plant-based AgNPs were synthesized using Raphanus sativus L. (RS) leaf aqua extract. Different concentrations of AgNO were used to optimize the synthesis process of RS-AgNPs from the aqueous leaf extract. Energy-dispersive X-ray analysis (EDX), transmission electron microscopy (TEM), scanning electron microscopy (SEM), atomic force microscope (AFM), and UV-vis spectroscopy were used to analyze the generated materials. Furthermore, to evaluate the biological properties of the obtained materials, Bacillus subtilis (B. subtilis), Pseudomonas aeruginosa (P. aeruginosa), Staphylococcus aureus (S. aureus), Escherichia coli (E. coli), and Candida albicans (C. albicans) pathogen strains were used for the minimum inhibitory concentration (MIC) assays. Subsequently, healthy cell lines (human dermal fibroblast (HDF)) and cancerous cell lines (glioma/U118, Ovarian/Skov-3, and colorectal adenocarcinoma/CaCo-2) were engaged to determine the cytotoxic effects of the synthesized NPs. The cytotoxic and anti-pathogenic potential of AgNPs synthesized by the proposed green approach was investigated. The results were encouraging compared to the standards and other controls. Plant-based AgNPs were found to be potential therapeutic agents against the human colon cancer cell (CaCo-2) and showed strong inhibitory activity on Candida albicans and Staphylococcus aureus growth. The RS-AgNPs generated have highly effective antimicrobial properties against pathogenic bacteria. Our findings also show that green RS-AgNPs are more cytotoxic against cancerous cell lines than normal cell lines. Synthesized nanoparticles with desirable morphology and ease of preparation are thought to be promising materials for antimicrobial, cytotoxic, and catalytic applications.
银纳米颗粒（AgNPs）有多种用途。许多科学家致力于从植物提取物中制备AgNPs，用作对抗耐药细菌和恶性细胞系的生物药物。在当前研究中，使用萝卜（Raphanus sativus L.，RS）叶水提取物合成了植物基AgNPs。使用不同浓度的硝酸银（AgNO₃）来优化从叶水提取物中合成RS-AgNPs的过程。采用能量色散X射线分析（EDX）、透射电子显微镜（TEM）、扫描电子显微镜（SEM）、原子力显微镜（AFM）和紫外可见光谱对生成的材料进行分析。此外，为了评估所得材料的生物学特性，使用枯草芽孢杆菌（B. subtilis）、铜绿假单胞菌（P. aeruginosa）、金黄色葡萄球菌（S. aureus）、大肠杆菌（E. coli）和白色念珠菌（C. albicans）病原体菌株进行最低抑菌浓度（MIC）测定。随后，使用健康细胞系（人皮肤成纤维细胞（HDF））和癌细胞系（胶质瘤/U118、卵巢癌/Skov-3和结肠腺癌/CaCo-2）来确定合成纳米颗粒的细胞毒性作用。研究了通过所提出的绿色方法合成的AgNPs的细胞毒性和抗病原潜力。与标准品和其他对照相比，结果令人鼓舞。发现植物基AgNPs是针对人结肠癌细胞（CaCo-2）的潜在治疗剂，并且对白色念珠菌和金黄色葡萄球菌的生长显示出强烈的抑制活性。生成的RS-AgNPs对病原菌具有高效的抗菌性能。我们的研究结果还表明，绿色RS-AgNPs对癌细胞系的细胞毒性比对正常细胞系更强。具有理想形态且易于制备的合成纳米颗粒被认为是用于抗菌、细胞毒性和催化应用的有前景的材料。
