Baran Ayşe, Fırat Baran Mehmet, Keskin Cumali, Hatipoğlu Abdulkerim, Yavuz Ömer, İrtegün Kandemir Sevgi, Adican Mehmet Tevfik, Khalilov Rovshan, Mammadova Afat, Ahmadian Elham, Rosić Gvozden, Selakovic Dragica, Eftekhari Aziz
Department of Biology, Mardin Artuklu University Graduate Education Institute, Mardin, Turkey.
Department of Medical Services and Techniques, Vocational School of Health Services, Mardin Artuklu University, Mardin, Turkey.
Front Bioeng Biotechnol. 2022 Mar 7;10:855136. doi: 10.3389/fbioe.2022.855136. eCollection 2022.
Using biological materials to synthesize metallic nanoparticles has become a frequently preferred method by researchers. This synthesis method is both fast and inexpensive. In this study, an aqueous extract obtained from chickpea ( L.) (CA) leaves was used in order to synthesize silver nanoparticles (AgNPs). For specification of the synthesized AgNPs, UV-vis spectrophotometer, Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction analysis (XRD), transmission electron microscopy (TEM), scanning electron microscopy (SEM), electron dispersive X-ray (EDX), and zeta potential (ZP) analyses data were used. Biologically synthesized AgNPs demonstrated a maximum surface plasmon resonance of 417.47 nm after 3 h. With the powder XRD model, the mean crystallite dimension of nanoparticles was determined as 12.17 mm with a cubic structure. According to the TEM results, the dimensions of the obtained silver nanoparticles were found to be 6.11-9.66 nm. The ZP of the electric charge on the surface of AgNPs was measured as -19.6 mV. The inhibition effect of AgNPs on food pathogen strains and yeast was determined with the minimum inhibition concentration (MIC) method. AgNPs demonstrated highly effective inhibition at low concentrations especially against the growth of (0.0625) and (0.125) strains. The cytotoxic effects of silver nanoparticles on cancerous cell lines (CaCo-2, U118, Sk-ov-3) and healthy cell lines (HDF) were revealed. Despite the increase of AgNPs used against cancerous and healthy cell lines, no significant decrease in the percentage of viability was detected.
利用生物材料合成金属纳米颗粒已成为研究人员经常首选的方法。这种合成方法既快速又廉价。在本研究中，为了合成银纳米颗粒（AgNPs），使用了从鹰嘴豆（L.）（CA）叶片中获得的水提取物。为了表征合成的AgNPs，使用了紫外可见分光光度计、傅里叶变换红外光谱（FT-IR）、X射线衍射分析（XRD）、透射电子显微镜（TEM）、扫描电子显微镜（SEM）、电子色散X射线（EDX）和zeta电位（ZP）分析数据。生物合成的AgNPs在3小时后表现出最大表面等离子体共振为417.47 nm。通过粉末XRD模型，确定纳米颗粒的平均微晶尺寸为12.17 mm，具有立方结构。根据TEM结果，发现获得的银纳米颗粒的尺寸为6.11 - 9.66 nm。AgNPs表面电荷的ZP测量值为-19.6 mV。用最小抑制浓度（MIC）法测定了AgNPs对食品病原菌菌株和酵母的抑制作用。AgNPs在低浓度下表现出高效抑制作用，尤其是对（0.0625）和（0.125）菌株的生长。揭示了银纳米颗粒对癌细胞系（CaCo-2、U118、Sk-ov-3）和健康细胞系（HDF）的细胞毒性作用。尽管用于癌细胞系和健康细胞系的AgNPs有所增加，但未检测到活力百分比有显著下降。
