Abo Zaid Mona H, El-Enany Nahed, Mostafa Aziza E, Hadad Ghada M, Belal Fathalla
Pharmaceutical Chemistry Department, Faculty of Pharmacy, Delta University for Science and Technology, Gamasa, 35712, Egypt.
Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Suez Canal University, Ismailia, 41522, Egypt.
BMC Chem. 2023 Mar 24;17(1):25. doi: 10.1186/s13065-023-00937-y.
Carbon quantum dots (CQDs) are new class of carbon nanoparticles. Recently, they have been widely used as fluorescent probes due to their easy accessibility, optical properties and chemical inertness. Many available precursors are used in the synthesis of carbon quantum dots. The electrical and optical properties of CQDs could be enhanced by doping hetero atoms such as nitrogen or sulfur into their structure.
The current work presents the synthesis and characterization of water-soluble nitrogen doped carbon quantum dots (N-CQDs) and their use as fluorescent nano-sensors for the spectrofluorimetric determination of furosemide in its pharmaceutical preparations and spiked human plasma.
A domestic microwave was used to prepare the N-CQDs by heating a solution of sucrose and urea till complete charring (about ten minutes). The produced N-CQDs exhibit a strong emission band at 376 nm after excitation at 216 nm. Furosemide caused a quantitative quenching in the fluorescence intensity of the produced N-CQDs.
The proposed method was validated according to ICH Guidelines. The method was found to be linear over the range of 0.1-1.0 µg/mL with LOQ of 0.087 µg/ml.
Ecofriendly nano fluorescent sensors (N-CQDs) were successfully synthesized. The size of N-CQDs was distributed in the range of 6.63 nm to 10.23 nm with an average of 8.2 nm. The produced N-CQDs were used as fluorescent probes for the estimation of furosemide in its pharmaceutical preparations as well as spiked human plasma samples.
碳量子点（CQDs）是一类新型碳纳米颗粒。近来，因其易于获取、光学性质及化学惰性，它们被广泛用作荧光探针。许多可用的前驱体被用于碳量子点的合成。通过将氮或硫等杂原子掺杂到碳量子点结构中，可增强其电学和光学性质。
当前工作展示了水溶性氮掺杂碳量子点（N-CQDs）的合成与表征，及其作为荧光纳米传感器用于荧光光谱法测定其药物制剂和加标人血浆中呋塞米的应用。
使用家用微波炉通过加热蔗糖和尿素溶液直至完全炭化（约十分钟）来制备N-CQDs。所制备的N-CQDs在216 nm激发后在376 nm处呈现出强发射带。呋塞米导致所制备的N-CQDs的荧光强度发生定量猝灭。
所提出的方法根据国际协调会议（ICH）指南进行了验证。该方法在0.1 - 1.0μg/mL范围内呈线性，定量限为0.087μg/ml。
成功合成了环境友好型纳米荧光传感器（N-CQDs）。N-CQDs的尺寸分布在6.63 nm至10.23 nm范围内，平均为8.2 nm。所制备的N-CQDs被用作荧光探针，用于测定其药物制剂以及加标人血浆样品中的呋塞米。
