Long Katherine D, Fu Jianing
Columbia Center for Translational Immunology, Department of Medicine, Columbia University Medical Center, New York, NY 10032, USA.
STAR Protoc. 2023 Mar 24;4(2):102192. doi: 10.1016/j.xpro.2023.102192.
Understanding immune cell dynamics after intestinal transplantation has provided new insights into human lymphocyte biology. However, isolating and characterizing such cells can be challenging. Here, we provide a protocol to isolate intraepithelial and lamina propria lymphocytes from human ileal biopsies. We describe techniques for flow cytometric analysis and determination of multilineage chimerism and T lymphocyte phenotypes. This protocol can be modified to isolate and analyze lymphocytes from other tissues. For complete details on the use and execution of this protocol, please refer to Fu et al. (2019) and Fu et al. (2021)..
对肠道移植后免疫细胞动态变化的理解为人类淋巴细胞生物学提供了新的见解。然而，分离和鉴定这些细胞可能具有挑战性。在这里，我们提供了一种从人回肠活检组织中分离上皮内和固有层淋巴细胞的方案。我们描述了流式细胞术分析以及多谱系嵌合体和T淋巴细胞表型测定的技术。该方案可进行修改以从其他组织中分离和分析淋巴细胞。有关该方案使用和执行的完整详细信息，请参考傅等人（2019年）和傅等人（2021年）的研究。
