College of Food Science, Northeast Agricultural University, Harbin, Heilongjiang 150030, China.
Sharable Platform of Large-Scale Instruments & Equipments, Northeast Agricultural University, Harbin, Heilongjiang 150030, China.
Ultrason Sonochem. 2023 May;95:106379. doi: 10.1016/j.ultsonch.2023.106379. Epub 2023 Mar 21.
Currently, as a promising alternative protein source, the interest of edible insect protein has been continuously increased. However, the extraction processing had distinct effects on the physicochemical properties and functionalities of this novel and sustainable protein. In this study, Tenebrio molitor larvae protein (TMLP) was extracted via ultrasound (US)-assisted alkaline extraction. The changes of extraction kinetics, physicochemical characteristics, and functional properties of TMLP as a function of US time (10, 20, 30, 40, 50 min) were investigated. The results showed that 30 min US treatment rendered the maximum protein yield (60.04 %) (P < 0.05). Meanwhile, Peleg's model was considered a suitable model to represent the extraction kinetics of TMLP, with a correlation coefficient of 0.9942. Moreover, the protein secondary structure, particle size, and amino acid profiles of TMLP were changed under the US-assisted alkaline extraction process. Additionally, a significant improvement of the functional properties of TMLP extracted with this method was observed compared to traditional alkaline extraction. In conclusion, the present work suggests that US-assisted alkaline extraction could be considered as a potential method to improve the protein yield, quality profiles, and functional properties of TMLP.
目前，作为一种有前途的替代蛋白质来源，食用昆虫蛋白的兴趣不断增加。然而，提取加工对这种新颖和可持续蛋白质的物理化学性质和功能有明显的影响。在这项研究中，采用超声（US）辅助碱性提取法提取了黄粉虫幼虫蛋白（TMLP）。研究了 US 时间（10、20、30、40、50 min）对 TMLP 提取动力学、物理化学特性和功能特性的影响。结果表明，30 min 的超声处理可获得最大的蛋白质产率（60.04%）（P < 0.05）。同时，认为 Peleg 模型适合表示 TMLP 的提取动力学，相关系数为 0.9942。此外，在超声辅助碱性提取过程中，TMLP 的蛋白质二级结构、粒径和氨基酸组成发生了变化。此外，与传统碱性提取相比，用这种方法提取的 TMLP 的功能特性得到了显著改善。总之，本研究表明，超声辅助碱性提取可以作为一种提高 TMLP 蛋白质产率、质量特性和功能特性的潜在方法。
