College of Food Science, Northeast Agricultural University, Harbin, Heilongjiang 150030, China; School of Food Engineering, Ludong University, Yantai, Shandong 264025, China.
Sharable Platform of Large-Scale Instruments & Equipments, Northeast Agricultural University, Harbin, Heilongjiang 150030, China.
Food Res Int. 2024 Nov;195:114974. doi: 10.1016/j.foodres.2024.114974. Epub 2024 Aug 23.
This work aimed to study the effect of defatting via the mixture of n-hexane and ethanol under different volume ratio on the changes of structural characteristics, functional properties and volatile compounds of Tenebrio molitor larvae protein (TMLP). The results showed that 1:0.6 vol ratio of n-hexane to ethanol rendered the highest defatting rate (P < 0.05), as well as led to the highest EAA/AA contents, sulfhydryl contents, surface hydrophobicity, solubility, water/oil holding capacities and emulsifying properties of TMLP (P < 0.05). However, higher volume ratio of n-hexane to ethanol led to negative impacts on functionalities of TMLP. Moreover, the contents of aldehydes and hydrocarbons which rendered off-flavour to TMLP significantly decreased with the increasing volume ratio of n-hexane to ethanol (P < 0.05), while the contents of pleasure flavour (hydrocarbons and ester compounds) were obviously enhanced. This study provides an eco-friendly defatting method on the processing of TMLP with superior quality attributes.
本研究旨在探讨正己烷和乙醇不同体积比混合物脱脂对黄粉虫幼虫蛋白（TMLP）结构特性、功能特性和挥发性化合物变化的影响。结果表明，正己烷与乙醇体积比为 1:0.6 时脱脂率最高（P < 0.05），同时导致 TMLP 的必需氨基酸/非必需氨基酸含量、巯基含量、表面疏水性、溶解度、水/油保持能力和乳化性能最高（P < 0.05）。然而，更高的正己烷与乙醇体积比会对 TMLP 的功能特性产生负面影响。此外，产生异味的醛类和碳氢化合物的含量随着正己烷与乙醇体积比的增加而显著降低（P < 0.05），而令人愉悦的风味（碳氢化合物和酯类化合物）的含量则明显增加。本研究为具有优良品质特性的 TMLP 加工提供了一种环保的脱脂方法。
