Department of Food Science and Technology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China.
State Key Laboratory of Dairy Biotechnology, Shanghai Engineering Research Center of Dairy Biotechnology, Dairy Research Institute, Bright Dairy & Food Co., Ltd., Shanghai 200436, China.
Food Chem. 2021 Feb 15;338:128158. doi: 10.1016/j.foodchem.2020.128158. Epub 2020 Sep 23.
Tenebrio molitor larvae protein isolates (TPIs) were extracted using the alkaline extraction and acid precipitation methods (AEAP) assisted by NaCl (salting-in) and (NH)SO (salting-out) procedures. The structural, physicochemical, and functional properties of TPIs were investigated. It was found that the salt-assisted treatments did not affect the total amino acid content but altered specific amino acid compositions. The salting-in-AEAP extraction resulted in non-significant (P > 0.05) differences in zeta potential, hydrophobicity, thermal stability, solubility and foaming capacity compared with the AEAP extraction. Salting-out-AEAP extraction significantly (P < 0.05) increased overall protein solubility, emulsion activity, foaming capacity and stability that were associated with lower hydrophobicity, higher zeta potential, α-helix and disulfide bond contents. The salting-in-AEAP-out extraction generated the greatest protein yield (39.54%), emulsion activity index (55.5 m/g), foaming capacity (205%) as well as foaming stability (65.59%).
利用碱性提取和酸沉淀法（AEAP），并辅以 NaCl（盐溶）和（NH）SO（盐析）程序，从黄粉虫幼虫蛋白分离物（TPIs）中提取蛋白质。研究了 TPIs 的结构、物理化学和功能特性。结果表明，盐辅助处理不会影响总氨基酸含量，但会改变特定氨基酸组成。与 AEAP 提取相比，盐溶-AEAP 提取对 Zeta 电位、疏水性、热稳定性、溶解度和泡沫能力没有显著差异（P > 0.05）。盐析-AEAP 提取显著提高了总蛋白质溶解度、乳化活性、泡沫能力和稳定性，这与较低的疏水性、较高的 Zeta 电位、α-螺旋和二硫键含量有关。盐溶-AEAP 提取的蛋白质得率（39.54%）、乳化活性指数（55.5 m/g）、泡沫能力（205%）和泡沫稳定性（65.59%）最高。
