Department of Pharmaceutical Chemistry, University of California San Francisco, San Francisco, CA 94158, USA; Biomedical Research Center (CINBIO), Department of Analytical and Food Chemistry, Campus Universitario de Vigo, University of Vigo, 36310 Vigo, Spain.
Department of Pharmaceutical Chemistry, University of California San Francisco, San Francisco, CA 94158, USA.
Food Chem. 2023 Aug 30;418:135960. doi: 10.1016/j.foodchem.2023.135960. Epub 2023 Mar 15.
Ciguatera Poisoning is an emerging risk in the east Atlantic Ocean. Despite characterization efforts, the complete profile of ciguatoxin chemical species in these waters is still unknown. These efforts have been complicated by a lack of reference materials and scarcity of fish contaminated with high levels of ciguatoxins. Development and application of analytical methods with enhanced selectivity and sensitivity is essential for ciguatoxin characterization. Here, we developed an analytical characterization approach using capillary liquid chromatography coupled to high resolution mass spectrometry applied to reference materials obtained from ciguatoxin contaminated fish. Capillary LC coupled mass spectrometry resulted in increased sensitivity leading to the confirmation of C-CTX1 as the principal ciguatoxin present in these samples. We also detected and structurally characterized minor C-CTXs analogues consisting of C-CTX3/4, hydroxy-, didehydro-, and methoxy- metabolites.
雪卡毒素中毒是东大西洋的一个新出现的风险。尽管进行了特征描述工作，但这些水域中雪卡毒素化学物质的完整特征仍不清楚。这些努力受到缺乏参考物质和高浓度雪卡毒素污染鱼类稀缺的阻碍。开发和应用具有增强选择性和灵敏度的分析方法对于雪卡毒素的特征描述至关重要。在这里，我们开发了一种使用毛细管液相色谱与高分辨率质谱联用的分析特征描述方法，应用于从雪卡毒素污染的鱼类中获得的参考物质。毛细管 LC 与质谱联用提高了灵敏度，从而确认 C-CTX1 是这些样品中主要存在的雪卡毒素。我们还检测并结构表征了由 C-CTX3/4、羟基、去氢和甲氧基代谢物组成的少量 C-CTX 类似物。
