Yon Thomas, Cruchet Philippe, Viallon Jérôme, Murray J Sam, Passfield Emillie, Chinain Mireille, Darius Hélène Taiana, Roué Mélanie
Institut Louis Malardé (ILM), UMR 241-SECOPOL (IFREMER, ILM, IRD, UPF), Laboratory of Marine Biotoxins, P.O. Box 30, 98713 Papeete, Tahiti, French Polynesia.
Cawthron Institute, 98 Halifax St East, PB 2, The Wood, Nelson 7010, New Zealand.
Mar Drugs. 2025 Jan 16;23(1):42. doi: 10.3390/md23010042.
Ciguatera poisoning (CP) is caused by the consumption of marine products contaminated with ciguatoxins (CTXs) produced by dinoflagellates of the genus . Analytical methods for CTXs, involving the extraction/purification of trace quantities of CTXs from complex matrices, are numerous in the literature. However, little information on their effectiveness for nonpolar CTXs is available, yet these congeners, contributing to the risk of CP, are required for the establishment of effective food safety monitoring programs. An evaluation of six extraction/purification protocols, performed with CTX3C spiked on fish flesh and a neuroblastoma cell-based assay (CBA-N2a), revealed recoveries from 6 to 45%. This led to the development of an optimized 3-day protocol designed for a large number of samples, with CTX1B and CTX3C eluting in a single fraction and showing recoveries of 73% and 70%, respectively. In addition, a reduction in adverse matrix effects in the CBA-N2a analyses was demonstrated with naturally contaminated specimens, increasing the sensitivity of the method, which now meets the very low guidance level recommended by international agencies. However, efforts are still required to reduce the signal suppression observed in LC-MS/MS analysis. This optimized protocol contributes to the technological advancement of detection methods, promoting food safety and improving CP risk assessment in marine products.
雪卡毒素中毒（CP）是由于食用了被甲藻属产生的雪卡毒素（CTXs）污染的海产品所致。文献中有许多关于CTXs的分析方法，这些方法涉及从复杂基质中提取/纯化痕量的CTXs。然而，关于它们对非极性CTXs有效性的信息很少，而这些同系物是导致CP风险的因素，对于建立有效的食品安全监测计划是必需的。对六种提取/纯化方案进行评估，将CTX3C添加到鱼肉中并采用基于神经母细胞瘤细胞的检测方法（CBA-N2a），回收率为6%至45%。这促使开发了一种针对大量样品的优化的三天方案，其中CTX1B和CTX3C在单一馏分中洗脱，回收率分别为73%和70%。此外，对于天然污染的样本，CBA-N2a分析中的不良基质效应有所降低，提高了该方法的灵敏度，该方法现在符合国际机构推荐的极低指导水平。然而，仍需要努力减少液相色谱-串联质谱分析中观察到的信号抑制。这种优化方案有助于检测方法的技术进步，促进食品安全并改善海产品中CP风险评估。
