Shao Chen, Lyu Jing, Li Tongxuan, Wang Jingyi, Lian Chunyu
Laboratory of Protozoological Biodiversity and Evolution in Wetland, College of Life Sciences, Shaanxi Normal University, Xi'an 710119, China.
Laboratory of Protozoological Biodiversity and Evolution in Wetland, College of Life Sciences, Shaanxi Normal University, Xi'an 710119, China.
Eur J Protistol. 2023 Apr;88:125970. doi: 10.1016/j.ejop.2023.125970. Epub 2023 Mar 8.
The morphology and morphogenesis of a new saline soil hypotrichous ciliate, Urosoma quadrinucleatum n. sp., collected from northwestern China, were studied based on live observations and protargol stained specimens. The new species is characterized as follows: size in vivo 90-130 × 20-30 μm; body outline elongate-elliptical with both ends broadly rounded; four macronuclear nodules; cortical granules present; paroral in front of endoral; usually 16 frontal-ventral-transverse cirri. Urosoma quadrinucleatum n. sp. has almost the same morphogenetic characteristics as its congeners U. gigantea and U. salmastra, and differs from other three congeners whose morphogenesis is known in the formation of the frontal-ventral-transverse cirral anlagen as well as the development of marginal and dorsal kineties anlagen. The sequence differences among U. quadrinucleatum n. sp. and other Urosoma species further support the validity of the present organism as novel species. Further, U. quadrinucleatum n. sp. clusters with U. salmastra in the phylogenetic analyses based on small subunit ribosomal RNA gene (SSU rDNA) sequence data.
基于活体观察和蛋白银染色标本，对从中国西北部采集的一种新的盐碱土腹毛目纤毛虫——四核尾柱虫（Urosoma quadrinucleatum n. sp.）的形态和形态发生进行了研究。新物种的特征如下：活体大小为90 - 130×20 - 30μm；身体轮廓呈长椭圆形，两端宽圆；有四个大核结节；有皮层颗粒；口前腔在口内膜前方；通常有16根额腹横纤毛。四核尾柱虫在形态发生特征上与其同属的巨大尾柱虫（U. gigantea）和盐沼尾柱虫（U. salmastra）几乎相同，在额腹横纤毛原基的形成以及边缘和背纤毛列原基的发育方面，与已知形态发生过程的其他三个同属物种不同。四核尾柱虫与其他尾柱虫物种之间的序列差异进一步支持了该生物作为新物种的有效性。此外，在基于小亚基核糖体RNA基因（SSU rDNA）序列数据的系统发育分析中，四核尾柱虫与盐沼尾柱虫聚类在一起。
