Poulsen Jan Struckmann, Nielsen Jeppe Lund
Department of Chemistry and Bioscience, Aalborg University, Fredrik Bajers Vej 7H, 9220, Aalborg E, Denmark.
Department of Chemistry and Bioscience, Aalborg University, Fredrik Bajers Vej 7H, 9220, Aalborg E, Denmark.
J Proteomics. 2023 May 15;279:104888. doi: 10.1016/j.jprot.2023.104888. Epub 2023 Mar 23.
Synthetic plastics, like polyethylene terephthalate (PET), have become an essential part of modern life. Many of these products are remarkably persistent in the environment, and the accumulation in the environment is recognised as a major threat. Therefore, an increasing interest has been focusing on the screening for organisms able to degrade and assimilate the plastic. Ideonella sakaiensis originally isolated from a plastisphere has been reported as a bacterium that was solely thriving on the degradation on PET films. The processes affected by the presence of PET and its monomeric substances terephthalic acid, ethylene glycol, ethyl glycolate, and sodium glyoxylate monohydrate were elucidated by analysis of differential protein expression. The exposure of PET and its monomers induced the MHETase and affect two major pathways: the TCA cycle and the β-oxidation pathway. The increased expression of proteins directly or indirectly involved in these pathways suggests their underlying importance in the degradation of PET by I. sakaiensis since these proteins are mechanistically supporting the enzymes involved in the degradation of PET and its monomers.
合成塑料，如聚对苯二甲酸乙二酯（PET），已成为现代生活的重要组成部分。这些产品中的许多在环境中具有很强的持久性，其在环境中的积累被认为是一个重大威胁。因此，人们越来越关注筛选能够降解和同化塑料的生物。最初从塑料球中分离出来的食烷菌属（Ideonella sakaiensis）被报道为一种仅靠降解PET薄膜生存的细菌。通过分析差异蛋白质表达，阐明了PET及其单体对苯二甲酸、乙二醇、乙醇酸乙酯和一水合乙醛酸钠的存在所影响的过程。PET及其单体的暴露诱导了MHETase，并影响两个主要途径：三羧酸循环和β-氧化途径。直接或间接参与这些途径的蛋白质表达增加，表明它们在食烷菌属降解PET过程中具有潜在重要性，因为这些蛋白质在机制上支持参与PET及其单体降解的酶。
